Figure 2.

The process of immune repertoire sequencing and analysis. The detailed steps of immune repertoire analysis are shown in the figure. The extraction and amplification of samples are the key techniques in library preparation. The starting materials that are either gDNA or mRNA have both advantages and disadvantages as templates. The choice of templates mainly depends on research purposes. gDNA was amplified by multiple pairs of primers, but there were large introns in the DNA, which led to amplification errors. The amplification for mRNA is operated by using 5'RACE which avoids PCR amplification bias, but the operation is more complicated. A large amount of data obtained from high-throughput sequencing of PCR products are used for health assessment, disease diagnosis, monitoring and prognosis.