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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Neurobiol Aging. 2021 Jun 20;106:241–256. doi: 10.1016/j.neurobiolaging.2021.06.012

Figure 2.

Figure 2.

Aged (19 months) and young (4 months) mice have equivalent tyrosine hydroxylase (TH) immunoreactive (-ir) cell counts across the rostrocaudal extent of the locus coeruleus. Aged mice have increased iba1-ir in the locus coeruleus. Photomicrographs depict representative TH-ir in the locus coeruleus at −5.62 mm bregma in A) young versus B) aged mice. White scale bars equal 100 micrometers. C) Line graph shows quantification of TH-ir cell counts. D-F) Photomicrographs show representative iba1-ir staining in D) young versus E) aged mice. Insets in C and D show higher magnification iba-ir staining. F) No primary antibody control for the iba1 channel in aged mice show age-related autofluorescence that is distinct from iba1-ir. Iba1-ir can be thresholded out above age-related background staining. G) Scatter bar graph depicts quantification of thresholded iba-ir % area in locus coeruleus averaged across 3 rostrocaudal levels.