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. Author manuscript; available in PMC: 2022 Sep 2.
Published in final edited form as: Mol Cell. 2021 Jul 27;81(17):3509–3525.e5. doi: 10.1016/j.molcel.2021.07.004

Figure 2. Detection of chromatin-independent nuclear insoluble RNAs.

Figure 2.

(A-C) RNA FISH for XIST and C0T-1 RNA signals after extraction of nuclei for 15 minutes as indicated (AS, ammonium sulfate). Scale bars 5 μm. (D,E) Immunofluorescence and RNA FISH of fixed cells grown on coverslips (top panels) or nuclei after serial extraction as indicated. Scale bars 10 μm.