Figure 5. Specific nuclear scaffold proteins are depleted from heterochromatin.

(A) Immunofluorescence of NUMA, MATR3, and SAF-A in TIG-1 fibroblasts. Higher magnification below. Line-scan of signal intensities for the indicated region shown at right. (B) DAPI intensity segmentation of the above nucleus. Numbers indicate high DAPI intensity bodies (yellow). (C) Log₂ ratio of the mean signal intensity within each indicated high intensity body compared to entire nucleus. (D) Log₂ ratio of the mean signal intensity within DAPI intensity segmentation classes compared to the entire nucleus. Error bars represent SEM of the log₂ ratio determined for multiple nuclei (n=4). (E) IF of NUMA, MATR3, and SAF-A in high passage (35) fibroblasts. Proteins were visibly depleted from high DAPI intensity bodies (SAHFs, arrows) in all cells with greater than 5 SAHFs (n=21). Higher magnification shown below and line-scan for the indicated region on right. Scale bars 5 μm