FIGURE 2.

PVT1 or AGO1 depletion decreases ovarian cancer cell viability and increases cell apoptosis. A, PVT1 expression was significantly decreased in SKOV3 ovarian cancer cell line where PVT expression was inhibited after two different siRNA transfection. B and C, AGO1 mRNA level (B) and protein expression (C) were also reduced significantly after siRNA targeting PVT1 transfection. D and E, The validity of siRNAs targeting AGO1 was verified by detecting the mRNA level (D) and protein expression (E) of AGO1 in SKOV3 cells. F, PVT1 level was determined in AGO1‐knockdown SKOV3 cells. G and H, Cell viability was measured using CCK‐8 assay in PVT1‐inhibited (G) and AGO1‐inhibited (H) cells. I, TUNEL assay was performed in transfected SKOV3 cells. The percentage of apoptotic nuclei (red) was calculated by normalizing total nuclei (blue). N = 4‐5 independent experiments. **P < .01, ***P < .001