FIGURE 4.

PVT1 directly targets miR‐148a‐3p. A and B, Potential miRNAs that target both AGO1 and PVT1 were predicted by the TargetScan, miRWalk and ENCORI databases. C, The expression of predicted miRNAs target AGO1 and PVT1 was determined through miRNA sequencing in SKOV3 cells transfected with siRNA targeting PVT1 or scramble. Heat map was drawn through TBtools v1.077 software. D, The expression of three most enriched miRNAs (miR‐148a‐3p, miR‐3361‐5p and miR‐15‐5p) was verified in different groups. E, The predicted miR‐148a‐3p binding sequence in PVT1 and the generation of dual‐luciferase reporter plasmids of wild‐type (WT) and mutant (MT) were shown. F and G, Luciferase activity assays were carried out in HER293T or SKOV3 cells cotransfected with miR‐148a‐3p mimic (F) or miR‐148a‐3p inhibitor (G) and PVT1 WT or PVT1 MT. N = 3 independent experiments. **P < .01, ***P < .001. ns indicates no significance