FIGURE 4.

MET alleviated oxidative stress‐induced senescence in HLE‐B3 cells. HLE‐B3 cells were treated with MET at different concentrations. (A). Representative images of SA‐β‐Gal staining of the cells. (B). Percentages of SA‐β‐Gal‐positive cells. (C). Relative fold‐changes in the mRNA levels of the genes encoding IL6, IL8, FAS, as determined by qRT‐PCR. (D). Relative fold‐changes in the mRNA levels of the genes encoding P21 and P16, as determined by qRT‐PCR. (E). Western blot analysis of P53, P21, p‐AMPKα (Thr172), p‐ACC(ser79) and β‐actin in HLE‐B3 cells treated with MET at different concentrations. Data were shown as mean ± SD and are representative of three independent experiments. *p < 0.05; **p < 0.01 compared to the indicated sample. The bar represents 50 um