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. 2021 Aug 23;12:717952. doi: 10.3389/fpls.2021.717952

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Copyright © 2021 Nakahira, Mizuno, Yamashita, Tsuchikura, Takeuchi, Shiina and Kawakami.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transplastomic plants expressing RGNNV-CP grown under photoautotrophic conditions. (A) Soil-grown wild-type (WT) and transplastomic (RGNNV1-1 and RGNNV1-2) plants were cultivated in a phytotron using sunlight and natural daylength, with a temperature cycle of 12 h at 26°C and 12 h at 23°C. Bar = 30 cm. (B) Mature leaves of WT and RGNNV1-2 plants. Bar = 5 cm. (C) Detection of RGNNV-CP accumulated in transplastomic plants (RGNNV1-1 and RGNNV1-2) by CBB staining. Total soluble protein (TSP) extracted from 1.5 mg of fresh leaf was separated by 13% SDS-PAGE gel and stained with CBB.