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. 2021 Aug 23;9:712627. doi: 10.3389/fcell.2021.712627

FIGURE 2.

FIGURE 2

Indirect Immunofluorescence of stress fibers in DITNC1 cells. (A) Cells were plated on coverslips and incubated with serum-free medium for 30 min, and then stimulated as indicated in Figure 1, for 5 min. Stress fibers were labeled with phalloidin/FITC (green). Magnification bar = 8 μm. Values in the graph are (B) Number of stress fibers per cell, (C) Average thickness (μm) of stress fibers per cell, and (D) Stress fiber coherency (alignment) per cell. Values in histograms and error bars represent mean + s.e.m. from at least 30 cells that were measured per condition in each experiment (n = 3). Statistical significance was calculated using Kruskal–Wallis non-parametric test with Dunn’s multiple comparison. MS, Mechanical Stress. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with basal conditions.