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. 2021 Aug 23;9:712627. doi: 10.3389/fcell.2021.712627

FIGURE 3.

FIGURE 3

Phosphorylation of Myosin Light Chain after Thy-1 stimulation, with or without mechanical stress. DITNC1 cells were incubated with serum-free medium for 30 min and stimulated as indicated in Figure 1 for 5, 10, 15, and 20 min. (A,C) Whole cell lysates were separated by SDS-PAGE and transferred to nitrocellulose for immunoblotting. Bands of 18 kDa correspond to Myosin Light Chain phospho-Serine 19 (p-MLC) and those at 90 kDa, to the Heat Shock Protein 90 (HSP90). Blue and red scissors indicate where the figure (immunoblot image) was cut so that bands match the order of sample appearance in the corresponding graphs. (B,D) Values in the graphs correspond to densitometric analysis of the p-MLC band intensity normalized to Heat Shock Protein 90 (HSP90) values. The application of mechanical forces exerted by a magnet are indicated using a gray shaded background in graph D. Statistical significance was calculated using Mann-Whitney non-parametric test. * and #, p < 0,05; *compared with the basal condition at T = 0, # compared with the value obtained at the same time with TRAIL-R2.