FIGURE 5.

Thy-1 plus mechanical stress increase surface levels of α_Vβ₃ Integrin faster than Thy-1 alone. (A) Forward and side scattering (FSC-H and SSC-A) gating shows the selected cell population, based on size and granularity. (B) The three cell populations shown correspond to non-labeled control (gray), isotype control (cyan) and α_Vβ₃ Integrin/Phycoerythrin (red). (C,D) DITNC cells were incubated with serum-free medium for 30 min and stimulated with Protein A-magnetic beads coated with Thy-1, with and without mechanical forces exerted with a magnet for 5, 10, 15, 30, and 45 min. Values in the graph are the mean fluorescence intensities normalized to the control signal (basal), TRAIL-R2 is a negative control in experiments with Thy-1. Values in the graph are the mean fluorescence intensities of α_Vβ₃ Integrin/Phycoerythrin, normalized to basal levels without stimuli. Values in the graphs represent mean + s.e.m. (n = 3). Statistical significance was calculated using Kruskal–Wallis non-parametric test, with Dunn’s multiple comparison. * and #, p < 0,05. * compared to the basal condition, # compared to TRAIL-R2 incubated for the same time.