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. 2021 Aug 23;12:724141. doi: 10.3389/fphar.2021.724141

FIGURE 1.

FIGURE 1

Administration of 3-MA inhibits autophagy and prevents peritoneal fibrosis in 4.25% PDF-induced rat model. (A) Photomicrographs show Masson’s trichrome staining of the peritoneum in each group. (B) Positive area of the Masson-positive submesothelial area (blue). (C) Thickness of the Masson-positive submesothelial area (blue). (D) Peritoneum tissue lysates were subjected to immunoblot analysis with specific antibodies against LC3, Beclin-1 and GAPDH. (E) Expression levels of LC3II and Beclin-1 were quantified by densitometry and normalized with LC3I and GAPDH respectively. (F) Photomicrographs show immunofluorescence co-staining of Beclin-1 and DAPI. Data are represented as the mean ± SEM. Means with different superscript letters are significantly different from one another (p < 0.05). All scale bars = 100 μm.