FIGURE 6.

3-MA inhibits activation of EGFR/ERK1/2 signaling pathway in vivo and in vitro. (A) Peritoneum tissue lysates were subjected to immunoblot analysis with specific antibodies against p-EGFR, EGFR, p-ERK1/2, ERK1/2 and GAPDH. (B, C) Expression levels of p-EGFR and p-ERK1/2 were quantified by densitometry and normalized with EGFR and ERK1/2 respectively. (D) Cell lysates were subjected to immunoblot analysis with specific antibodies against p-EGFR, EGFR, p-ERK1/2, ERK1/2 and GAPDH. (E) Expression levels of p-EGFR and p-ERK1/2 were quantified by densitometry and normalized with EGFR and ERK1/2 respectively. Data are represented as the mean ± SEM. Means with different superscript letters are significantly different from one another (p < 0.05).