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. 2021 Aug 23;12:724141. doi: 10.3389/fphar.2021.724141

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Copyright © 2021 Shi, Hu, Wang, Ma, Tang, Tao, Qiu, Zhuang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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3-MA suppresses peritoneal angiogenesis through inhibiting β-catenin signaling pathway. (A) Photomicrographs illustrate immunohistochemistry staining of CD31, CD34 and VEGF from peritoneal tissues. (B-D) The count of CD31-positive vessels, CD34-positive vessels and VEGF-positive cells. (E) Cell lysates were subjected to immunoblot analysis with specific antibodies against β-catenin and GAPDH. (F) Expression level of β-catenin was quantified by densitometry and normalized with GAPDH. Data are represented as the mean ± SEM. Means with different superscript letters are significantly different from one another (p < 0.05). All scale bars = 100 μm.