FIGURE 1.

Experimental design of the in vivo study. The db/m and db/db mice were housed at 22 ± 2°C, 40–70% relative humidity and 12-h light/dark cycle. After 7 days, RBG was measured by snipping the tails. The diabetic mice were randomly divided into Model, AOS (380 or 750 mg/kg/day), and Metformin (160 mg/kg/day) groups. The drug treatment lasted 8 weeks. RBG levels were measured weekly during 8 weeks of AOS and metformin treatment. At the end of the experiments, the mouse were sacrificed for further experiments.