Table 1.
Promiscuous B-cell epitopes from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) unannotated open reading frame (uORF) proteins are listed along with the highly recurring (HR) or moderately recurring (MR) mutations, if any.
| uORF proteins | Positions | Epitope | Mutation (HR/MR) | Percentage frequency (%) |
|---|---|---|---|---|
| Linear B-cell epitopes | ||||
| 1auORF2.ext | 22–29 | STSRFRPG | R27C | 98.99 |
| M.ext | 196–202 | ASQRVAG | ||
| 213–230 | RIGNYKLNTDHSSSSDNI | |||
| ORF3c | 14–22 | YRYKPHSLS | L21F; K17E | 1.58; 1.25 |
| 7a.iORF1 | 34–47 | SSGTYEGNSPFHPL | ||
| 74–83 | QLRARSVSPK | |||
| N.iORF2 | 22–32 | AVGRDQNNVGP | ||
| ORF9b | 5–10 | ISEMHP | ||
| 29–39 | AVGRDQNNVGP | |||
| 65–69 | EDKAF | |||
| Conformational B-cell epitopes | ||||
| ORF9b (PDB ID:6Z4U) | 61–68 | LNSLEDKA | ||
Note that the residue numbers of an epitope (column 2) located in the particular protein (column 1) and its amino acid sequence (column 3) are given along with the (if any) HR/MR (column 4) mutation(s) present in the epitope. Note that column 5 represents the percentage frequency of the HR/MR. For the uORF HR/MR mutations, refer to Figure 4.