Table 2.
Promiscuous CD8+ T-cell epitopes from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) unannotated open reading frame (uORF) proteins are listed along with the number of human leukocyte antigen (HLA) I alleles.
| uORF proteins | Positions | Epitopes | No. of alleles | CD8+ immunogenicity score | Vaxijen antigenicity score | Population coverage of the alleles | HR/MR mutation | Percentage frequency (%) |
|---|---|---|---|---|---|---|---|---|
| ORF3c | 28–36 | ALHFLLFFR | 5 | 0.27426 | 0.7293 | 29.31% | R36I | 20.18 |
| 6.iORF | 5–13 | KVSIWNLDY | 9 | 0.29343 | 0.8195 | 59.07% | ||
| 7a.iORF1 | 96–104 | SPIFLIVAA | 6 | 0.33318 | 0.6265 | 33.20% | ||
| 99–107 | FLIVAAIVF | 7 | 0.29611 | 0.6203 | 24.47% | |||
| 102–110 | VAAIVFITL | 28 | 0.43202 | 0.8679 | 100.00% | |||
| 7b.iORF1 | 3–11 | IIFWFSLEL | 17 | 0.2683 | 0.8291 | 99.98% | ||
| M.ext | 89–97 | ITGGIAIAM | 11 | 0.34671 | 0.7715 | 54.64% | ||
| 63–71 | KLIFLWLLW | 5 | 0.34287 | 0.4968 | 18.61% | |||
| 19–27 | GTITVEELK | 4 | 0.29473 | 1.0976 | 37.50% | |||
| 151–159 | LVIGAVILR | 7 | 0.2601 | 1.1027 | 46.67% | |||
| 149–157 | SELVIGAVI | 11 | 0.25658 | 0.6409 | 43.14% | |||
| ORF9b | 41–48 | KVYPIILRL | 59 | 0.2634 | 0.6489 | 100.00% | ||
| 87–95 | LPDEFVVVT | 6 | 0.31977 | 0.5057 | 24.2% |
Note that the residue numbers of an epitope (column 2) located in the particular protein (column 1) and its amino acid sequence (column 3) are given along with its immunogenicity (column 5) and antigenicity (column 6) scores. Note that the number of HLA I alleles to which the epitope binds with (column 4) is also given along with the population coverage of the alleles (column 7). Column 8 represents the highly recurring (HR)/moderately recurring (MR) mutation(s) present in the epitope, if any. The percentage frequencies of the HR/MR mutation(s) are given in column 9. For the uORF HR/MR mutations, refer to Figure 4.