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. 2021 Aug 6;22(9):e52247. doi: 10.15252/embr.202052247

Figure EV1. Loss of miR‐183 and miR‐96 enhances muscle oxidative phenotype, related to Fig 1 .

Figure EV1

  • A
    Relative expression of miR‐182 mRNA in GAS muscles, liver and inguinal WAT of WT and DKO mice (n = 5).
  • B
    HE staining of GAS muscles of WT and DKO mice (left). The total fiber number of GAS muscle was determined (right, n = 5).
  • C
    Relative mRNA expression of MyoD and Myf5 in GAS muscles of WT and DKO mice (n = 5).
  • D
    Relative expression of MHC isoforms mRNA in TA muscles of WT and DKO mice (n = 2 or 4).
  • E
    Whole‐body oxygen consumption rate (left) and energy expenditure (middle) of WT and DKO mice in light and dark phases (n = 5). Energy expenditure rate of WT and DKO mice in light and dark phases (n = 5).
  • F
    Relative mRNA expression of PGC1α and PLIN5 in SOL muscles of WT and DKO mice (n = 3).
  • G
    Relative mRNA expression of fatty acid oxidation (FAO)‐related genes in GAS muscles of WT and DKO mice (n = 4 for WT; n = 5 for DKO).

Data information: Means ± SEM are shown for all panels. *P < 0.05; **P < 0.01; NS, not significant (Student’s t‐test). All experiments were performed at least three times, and representative data are shown.Source data are available online for this figure.