miR‐183 and miR‐96 orchestrate both glucose and fat utilization in skeletal muscle

A, B

Random (A) and fasting (B) blood glucose levels of WT and DKO mice (n = 8 for DKO fasted group, n = 10 for all other groups).

C

Glucose tolerance test (GTT) and area under the curve (AUC) data for GTT in WT and DKO mice (n = 8 for DKO, n = 10 for WT).

D

HE staining of the iWAT and eWAT of WT and DKO mice. Scale bars: 100 μm.

E

Analysis of fat mass of WT and DKO mice by NMR technique (n = 7) (left) and tissue weight of iWAT, eWAT, and iBAT (right) (n = 4).

F

Serum levels of triglycerides (TAG) and non‐esterified free fatty acids (NEFA) in WT and DKO mice (n = 5).

G

Respiratory exchange ratio (RER) of WT and DKO mice in light and dark phases (n = 5).

H

Relative mRNA expression of FoxO1 and PDK4 in GAS muscles of WT and DKO mice (n = 5).

I

Western blot analysis of FoxO1, PDK4, p‐PDHA1, and PDHA1 in GAS muscles of WT and DKO mice.

J

Western blot analysis of FoxO1, PDK4, p‐PDHA1, and PDHA1 in C2C12 cells transfected with Ago‐183/96 (left) or Ant‐183/96 (right).

K

Relative mRNA expression of ATGL and HSL in GAS muscles of WT and DKO mice (n = 5).

L

Western blot analysis of HSL and ATGL in GAS muscles of WT and DKO mice.

M

Western blot analysis of HSL and ATGL in C2C12 cells transfected with Ago‐183/96 (left) or Ant‐183/96 (right).

N–Q

LD area per cell was quantified at the indicated time points to measure LD turnover in C2C12 myocytes transfected with agomiR‐183/96 (N) or antagomir‐183/96 (P) stained with Nile red (n = 5). Representative immunofluorescence images of lipid droplets in C2C12 cells transfected with agomiR‐183/96 (O) or antagomir‐183/96 (Q). Scale bars: 5 μm.

Figure 2. Loss of miR‐183 and miR‐96 alters glucose and lipid homeostasis and fuel metabolism in skeletal muscle.