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. 2021 Aug 6;22(9):e52247. doi: 10.15252/embr.202052247

Figure EV2. Loss of miR‐183 and miR‐96 alters glucose and lipid homeostasis and fuel metabolism in skeletal muscle, related to Fig 2 .

Figure EV2

  • A
    The serum insulin levels of WT and DKO mice were determined (n = 5).
  • B
    Pyruvate tolerance test (PTT) were performed in WT mice and DKO mice (n = 10 for WT; n = 12 for DKO) (left). Area under the curve (AUC) data for PTT tests were calculated (right).
  • C
    Relative mRNA expression of gluconeogenic genes in the liver of WT and DKO mice (n = 5).
  • D
    Relative mRNA expression of thermogenic genes in the BAT of WT and DKO mice (n = 5).
  • E
    Food intake (left) and physical activity (right) of WT and DKO mice (n = 5). Light: light phase; Dark: dark phase.
  • F, G
    Respiratory exchange ratio (RER) and mean ΔRER of WT and DKO mice in light and dark phases (n = 5).
  • H
    Relative mRNA expression of lipogenic genes in the liver of WT and DKO mice (n = 5).
  • I
    HE staining of the liver of WT and DKO mice. Scale bar: 100 μm.
  • J
    Western blot analysis of FoxO1, PDK4, p‐PDHA1, and PDHA1 in SOL muscles of WT and DKO mice (left). Western blot analysis of HSL and ATGL in SOL muscles of WT and DKO mice (right).
  • K
    Relative mRNA expression of glycolytic genes in GAS muscles of WT and DKO mice (n = 5).
  • L
    Lactate, pyruvate, and LDHA mRNA levels were determined in the GAS muscles of WT and DKO mice (n = 5).
  • M
    Lactate, pyruvate, and LDHA mRNA levels were determined in the C2C12 cells treated with Ago‐183/96 or Ant‐183/96 (n = 5).
  • N, O
    qPCR analysis of relative miR‐183 (N) and miR‐96 (O) levels in different tissues (Ep, epididymal fat; Kid, kidney; Liv, liver; Lun, lung; Mus, skeletal muscle; Pan, pancreas; Spl, spleen) from C57BL/6J mice as indicated (n = 3).
  • P, Q
    Relative mRNA expression (P) and Western analysis (Q) of FoxO1, PDK4, ATGL, HSL, and PLIN5 in the inguinal WAT of WT and DKO mice (n = 5).
  • R, S
    Relative mRNA expression (R) and Western analysis (S) of FoxO1, PDK4, ATGL, HSL, and PLIN5 in the liver of WT and DKO mice (n = 5).

Data information: Means ± SEM are shown for all panels. *P < 0.05; NS, not significant (Student’s t‐test). All experiments were performed at least three times, and representative data are shown.Source data are available online for this figure.