Figure 4. miR‐183 and miR‐96 modulate fat usage via FoxO1 and ATGL in muscle cells.

• A
  Relative mRNA expression of ATGL, PGC1α, PLIN5, and HSL in C2C12 cells transfected with ATGL (n = 5).
• B
  Relative mRNA expression and Western blot analysis of ATGL in C2C12 cells transfected with Ant‐183/96 and siRNA of ATGL (siATGL) as indicated (n = 3 per group for RT–PCR analysis).
• C, D
  Relative mRNA expression (C) and Western blot analysis (D) of PGC1α, PLIN5, and HSL in C2C12 cells transfected with Ant‐183/96 and siATGL as indicated (n = 3 per group for RT–PCR analysis).
• E, F
  Relative mRNA expression of ATGL (E) and Western blot analysis (F) of FoxO1 and ATGL in C2C12 cells transfected with Ago‐183/96 and FoxO1 as indicated (n = 5 per group for RT–PCR analysis).
• G, H
  Relative mRNA expression (G) and Western blot analysis (H) of PGC1α, PLIN5, and HSL in C2C12 cells transfected with Ago‐183/96 and FoxO1 as indicated (n = 5 per group for RT–PCR analysis).
• I
  Relative mRNA expression of PGC1α, PLIN5, and HSL in C2C12 cells transfected with Ant‐183/96 and siRNA of PPARδ (siPPARδ).

Data information: Means ± SEM are shown for all panels. *P < 0.05 versus control; **P < 0.01 versus control; ***P < 0.001 versus control (Student’s t‐test). The loading control was reused in Fig 4F and H. All experiments were performed at least three times, and representative data are shown.

Source data are available online for this figure.