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. 2021 Aug 6;22(9):e52247. doi: 10.15252/embr.202052247

Figure 5. miR‐183 and miR‐96 levels are low in oxidative muscles and downregulated upon HFD feeding.

Figure 5

  1. Relative expression of miR‐183 and miR‐96 mRNAs in GAS and SOL muscles of C57 BL/6 mice (n = 3).
  2. Relative mRNA expression of ATGL, HSL, and PLIN5 in GAS and SOL muscles of C57 BL/6 mice (n = 3).
  3. Western blot analysis of ATGL, HSL, and PLIN5 in GAS and SOL muscles of C57 BL/6 mice.
  4. Relative expression of miR‐183 and miR‐96 mRNAs in GAS muscles of C57 BL/6 mice fed with HFD for 2 months (n = 5).
  5. Relative mRNA expression of ATGL, HSL, and PLIN5 in GAS muscles of C57 BL/6 mice fed with HFD for 2 months (n = 5).
  6. Western blot analysis of ATGL, HSL, and PLIN5 in GAS muscles of C57 BL/6 mice fed with HFD for 2 months.
  7. The role of skeletal muscle miR‐183 and miR‐96 in fuel metabolism. The effects of miR‐183 and miR‐96 deficiency (genetic ablation) and downregulation (HFD feeding), and low levels of miR‐183 and miR‐96 (more oxidative myofibers) on gene expression, enzyme, or transcription factor activity, and metabolic pathways involved in fuel usage are shown by up/down arrows or cross in red.

Data information: Means ± SEM are shown for all panels. *P < 0.05 versus control; **P < 0.01 versus control; ***P < 0.001 versus control (Student’s t‐test). All experiments were performed at least three times, and representative data are shown.

Source data are available online for this figure.