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. 2021 Jul 1;22(9):e52547. doi: 10.15252/embr.202152547

Figure 1. Type I IFN induces the formation of PML‐NBs in primary peripheral neurons.

Figure 1

  • A
    Representative images of primary neurons isolated from superior cervical ganglia (SCG) and sensory trigeminal ganglia (TG) of postnatal (P6) and adult (P28) mice stained for PML and the neuronal marker BIII‐tubulin.
  • B
    SCG and TG neurons isolated from P6 and P28 mice were treated with interferon (IFN)α (600 IU/ml) for 18 h and stained for PML and BIII‐tubulin.
  • C–F
    Quantification of detectable PML puncta in P6 and P28 neurons following 18‐h treatment with IFNα (150 IU/ml, 600 IU/ml), IFNγ (150 IU/ml, 500 IU/ml), and IFNλ2 (100 ng/ml, 500 ng/ml). Data information: Data represent the mean ± SEM. n = 60 cells from 3 biological replicates. Statistical comparisons were made using a one‐way ANOVA with Tukey’s multiple comparison (ns not significant, ****P < 0.0001). Scale bar, 20 μm.

Source data are available online for this figure.