Figure 6. Depletion of PML with shRNA‐mediated knockdown prior to infection restores HSV‐1 reactivation in type I interferon‐treated primary sympathetic neurons.

• A
  Quantification of PML puncta in P6 SCG neurons transduced with either control non‐targeting shRNA or shRNA targeting PML for 3 days, then treated with IFNα (600 IU/ml) for 18 h. n = 60 cells from 3 biological replicates.
• B
  Quantification of colocalization of vDNA foci detected by click chemistry to PML in primary SCG neurons transduced with shRNA targeting PML for 3 days prior to being infected with HSV‐1^EdC in the presence or absence of IFNα (150 IU/ml). Each point represents the percentage of 20 vDNA foci that colocalized to PML from 3 biological replicates.
• C–F
  Number of Us11‐GFP expressing neurons at 3 days post‐LY294002‐induced reactivation in P6 SCG neuronal cultures transduced with shRNA targeting PML for 3 days prior to infection with HSV‐1 in the presence or absence of IFNα (150 IU/ml). n = 9 biological replicates.

Data information: Data represent the mean ± SEM. Statistical comparisons were made using a one‐way ANOVA with Tukey’s multiple comparison (ns not significant, **P < 0.01, ****P < 0.0001).

Source data are available online for this figure.