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. 2021 Jul 20;22(9):e52252. doi: 10.15252/embr.202052252

Figure 5. SOCS6 regulates the expression of chemokines via modulating JAK/STAT signaling.

Figure 5

  • A
    ELISA was performed to detect the secretion of cytokines TNF‐α, IL‐1, IL‐6, and CXCL15 in Socs6 +/+ and Socs6 −/− BMDMs during Mtb stimulation. Data are shown as the mean ± SEMof n = 3 biological replicates.
  • B
    Phosphorylation states of STAT1 in response to Mtb stimulation in Socs6 −/− BMDMs were examined by Western blotting. Fludarabine treatment concentration was 10 μM, and the treatment time was 24 h. Representative blots from n = 3 biological replicates are shown.
  • C–F
    Relative expressions of chemokines CCL5, CXCL10, ICAM1 (C), and caspase 3, caspase 7, caspase 8 (F) in Mtb‐stimulated Socs6 +/+ or Socs6 −/− BMDMs were detected by qRT–PCR. Data are shown as the mean ± SEM of n = 3 biological replicates. Cluster analysis of clinical samples. Heat maps showed relatively high (red) or low (green) expression levels of Socs6 and inflammatory factors in PBMCs from healthy individuals (n = 27) (D) and TB patients (n = 34) (E).

Data information: ANOVA followed by Bonferroni post hoc test (A, C, F) was used for data analysis. **P < 0.01. Abbreviation: n.s., not significant.

Source data are available online for this figure.