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. 2021 Aug 12;22(9):e52576. doi: 10.15252/embr.202152576

Figure EV1. Conditional knockout of Cdc20 impairs bone formation and blunts bone regeneration, related to Fig 1 .

Figure EV1

  1. The design strategy of conditional deletion of Cdc20 gene.
  2. Representative image of PCR genotypes of indicated mice. Sp7‐Cre;Cdc20f / f mice were in experimental groups, Cdc20f / f mice were in control groups.
  3. Representative micro‐CT images and H&E staining of trabecular bone from the femoral metaphysis of 12‐week‐old male Sp7‐Cre;Cdc20f / f and littermate control mice. Scale bar, 500 μm.
  4. Histomorphometric analyses of 12‐week‐old male femurs (n = 6).
  5. Representative micro‐CT images and H&E staining of trabecular bone from the femoral metaphysis of 12‐week‐old female Sp7‐Cre;Cdc20f / f and littermate control mice. Scale bar, 500 μm.
  6. Histomorphometric analyses of 12‐week‐old female femurs (n = 6).

Data information: Data are displayed as mean ± SD and show one representative of n ≥ 3 independent experiments with three biological replicates. Statistical significance was calculated by a two‐tailed unpaired Student’s t‐test and defined as *P < 0.05, **P < 0.01.