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. 2021 Aug 12;22(9):e52576. doi: 10.15252/embr.202152576

Figure 4. CDC20 interacts with p65.

Figure 4

  • A, B
    Co‐immunoprecipitation of ectopically expressed Myc‐CDC20 with ectopically expressed Flag‐p65 in HEK293T cells.
  • C, D
    Co‐immunoprecipitation of ectopically expressed Myc‐CDC20 with endogenous p65 and ectopically expressed Flag‐p65 with endogenous CDC20 in HEK293T cells.
  • E, F
    Co‐immunoprecipitation of endogenous CDC20 with endogenous p65 in HEK293T cells.
  • G
    Co‐immunoprecipitation of purified GST‐CDC20 protein with endogenous p65 in HEK293T cells and the construct of GST‐CDC20. Whole‐cell lysates (WCL) were subjected to perform GST pull‐down analyses with purified GST‐CDC20 and GST as a negative control.
  • H
    Co‐immunoprecipitation of purified GST‐p65 protein with endogenous CDC20 in HEK293T cells and the construct of GST‐p65. Whole‐cell lysates (WCL) were subjected to perform GST pull‐down analyses with purified GST‐p65 and GST as a negative control.