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. 2021 Aug 12;22(9):e52576. doi: 10.15252/embr.202152576

Figure EV4. CDC20 regulates p65 degradation in an APC11‐dependent manner, related to Fig 6 .

Figure EV4

  • A, B
    No interaction was found in the immunoprecipitation of p65 and APC2 in HEK293T cells.
  • C, D
    The expression of p65 remained stable under the knockdown (C) or overexpression (D) of APC2 in HEK293T cells determined by Western blot analyses.
  • E, F
    No change of p65 protein (E) and CDC20 protein (F) were seen in NC and CDH1sh HEK293T cells.
  • G
    The interaction of APC11 and p65 remained stable in NC and CDH1sh HEK293T cells.
  • H
    The knockdown efficiency of APC11 in hBMSCs was determined by qRT–PCR (n = 6).

Data information: Data are displayed as mean ± SD and show one representative of n ≥ 3 independent experiments with three biological replicates. Statistical significance was calculated by one‐way ANOVA followed by a Tukey’s post hoc test and defined as ***P < 0.001.