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. 2021 Aug 12;22(9):e52576. doi: 10.15252/embr.202152576

Figure 5. The functional domain of CDC20 and p65.

Figure 5

  • A, B
    Co‐immunoprecipitation of ectopically expressed Myc‐CDC20 171–499 fragment (containing WD40 domain) (A) rather than Myc‐CDC20 1–170 fragment (lacking WD40 domain) (B) with endogenous p65 in HEK293T cells.
  • C
    Schematic representation of RHD domain location in p65 peptides (amino acids 19‐306).
  • D
    Western blot analyses of Flag‐p65, Flag‐p65 1–310 fragment (containing RHD domain), Flag‐p65 311–551 fragment (lacking RHD domain) plasmids expression in HEK293T cells.
  • E, F
    Co‐immunoprecipitation of ectopically expressed Flag‐p65 1–310 fragment (containing RHD domain) (E) rather than Flag‐p65 311–551 fragment (lacking RHD domain) (F) with endogenous CDC20 in HEK293T cells.
  • G, H
    Co‐immunoprecipitation of ectopically expressed Myc‐CDC20 171–499 with ectopically expressed Flag‐p65 1–310 fragment in HEK293T cells.