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. 2021 Aug 12;22(9):e52576. doi: 10.15252/embr.202152576

Figure EV5. CDC20 regulated osteogenic differentiation of BMSCs in a p65‐dependent manner, related to Fig 8 .

Figure EV5

  • A
    Fluorescent staining of lentiviruses injected from tail intravenously.
  • B, C
    The efficiency of p65 knockdown determined by qRT–PCR (B) and Western blot (C) of BMSCs in Sp7‐Cre;Cdc20f / f mice (n = 6).
  • D, E
    The ALP staining (D) and ALP quantification (E) of control and CDC20 knockdown hBMSCs after 7 days osteogenic differentiation treated with negative control or p65si RNA (n = 5).
  • F, G
    The ALP staining (F) and ALP quantification (G) of NC and CDC20sh hBMSCs after 7 days osteogenic differentiation treated with BAY 11–7,082 (n = 6).
  • H
    The expression of RUNX2 in BMSCs from Cdc20f / f mice and Sp7‐Cre;Cdc20f / f mice after 7 days osteogenic differentiation treated with negative control or p65si, determined by qRT–PCR (n = 5).

Data information: Data are displayed as mean ± SD and show one representative of n ≥ 3 independent experiments with three biological replicates. Statistical significance was calculated by one‐way ANOVA followed by independent two‐tailed Student’s t‐tests or a Tukey’s post hoc test and defined as *P < 0.05, **P < 0.01, ***P < 0.001.