Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models	Source (public):
C57BL/6 (M. musculus)	Beijing Biocytogen Co., Ltd	C57BL/6‐Sp7tm1(iCre)/Bcgen
hBMSCs	ScienCell Research Laboratories	Cat #7500
Recombinant DNA
MT‐CDC20	Addgene	Cat #11593
pcDNA3‐HA‐APC11	Addgene	Cat #19898
pBlueBac human APC2	Addgene	Cat #11425
pRSV‐p65	Addgene	Cat #20994
Antibodies
Rabbit anti‐α‐tubulin	Proteintech	Cat #11224‐1‐AP
Rabbit anti‐GAPDH	Proteintech	Cat #10494‐1‐AP
Rabbit anti‐CDH1	Proteintech	Cat #20874‐1‐AP
Rabbit anti‐Ubiquitin	Proteintech	Cat #10201‐2‐AP
Rabbit anti‐OCN	Proteintech	Cat #23418‐1‐AP
Rabbit IgG	Proteintech	Cat #B900610
Mouse IgG	Proteintech	Cat #B900620
HRP‐Mouse Anti‐Rabbit IgG Light Chain Specific	Proteintech	Cat #SA00001‐7L
Mouse anti‐HA‐Tag	Proteintech	Cat #66006‐1‐Ig
Mouse anti‐His‐Tag	Proteintech	Cat #66005‐1‐Ig
IPKine HRP AffiniPure Goat Anti‐Mouse IgG Light Chain	Abbkine	Cat #A25012
Rabbit anti‐p65	Abcam	Cat #ab16502
Mouse anti‐p65	Cell Signaling Technology	Cat #6956T
Rabbit anti‐Cyclin B1	Abcam	Cat #ab181593
Rabbit anti‐CDC20	Abcam	Cat #ab183479
Rabbit anti‐IκBα	Abcam	Cat #ab32518
Goat anti‐mouse	Abcam	Cat #ab6789
Goat anti‐rabbit	Abcam	Cat #ab190492
Rabbit anti‐RUNX2	Cell Signaling Technology	Cat #12556
Rabbit anti‐H3	Cell Signaling Technology	Cat #2650S
Rabbit anti‐APC2	Cell Signaling Technology	Cat #12301S
Rabbit anti‐APC11	Cell Signaling Technology	Cat #14090
Mouse anti‐Flag‐tag	Cell Signaling Technology	Cat #8146
Mouse anti‐Myc‐tag	Cell Signaling Technology	Cat #2276
Rabbit anti‐GST	Cell Signaling Technology	Cat #2622
Rabbit anti‐phospho‐p65	Cell Signaling Technology	Cat #3033
Goat anti‐mouse	ZSGB‐BIO	Cat # ZF‐0313
Goat anti‐rabbit	ZSGB‐BIO	Cat # ZF‐0311
Rabbit anti‐COL1A1	Serviebio	Cat #GB11022‐1
Rabbit anti‐COL2A1	Serviebio	Cat #GB11021
Rabbit anti‐OPN	Serviebio	Cat #GB11500
Oligonucleotides and other sequence‐based reagents
PCR primers	This study	Table 2
shRNA sequences	This study	Table 2
siRNA sequences	This study	Table 2
sgRNA sequences	This study	Table 1
Chemicals, Enzymes and other reagents
Recombinat Human TNF‐α Protein	R&D	Cat #210‐TA‐020
MG132	APE×BIO	Cat #A2585
BAY 11‐7082	Selleck	Cat #S2913
Software
GraphPad software	www.graphpad.com
ImageJ	https://imagej.net/ImageJ
Other