Figure 3. The NimB4^sk2 mutant is defective in cell corpse clearance.

1. Representative confocal imaging of wild‐type (w¹¹¹⁸), NimB4^sk2 , and apoptosis‐null NimB4^sk2; Df(3L) H99 embryonic macrophages at stage 16 of development; ventral and lateral view; Tissues were co‐stained with Dcp‐1 (red, corresponding to apoptotic corpses) and anti‐SIMU (green, corresponding to macrophages) antibodies. The arrows show the presence of apoptotic cells inside embryonic macrophages. Scale bars = 20 μm.
2. Quantification of caspase‐positive particles (anti‐Dcp‐1, red) within macrophages (anti‐SIMU, green). Values from at least five independent experiments are represented as mean ± SD total volume of caspase‐positive particles (**P < 0.01, by Mann–Whitney test).
3. Representative projections from confocal stacks of entire third‐instar wild‐type (w¹¹¹⁸) and NimB4^sk2 larval brains stained with anti‐Draper (green) and anti‐Dcp‐1 (red). The arrows show the presence of apoptotic cells in the central area of NimB4^sk2 larval brain. Scale bars = 100 μm.
4. Quantification of caspase‐positive particles in the central brain area of larval brain. Values from at least five independent experiments are represented as mean ± SD total volume of caspase‐positive particles (**P < 0.01, by Mann–Whitney test).