TABLE 1.
Probes used for fluorescence detection of DNA
Probe | Nucleotide sequence (5′ to 3′) and chemistrya |
---|---|
All-CAN-TET | 5′-TET AG GGC ATG CCT GTT TGA GCG TC(GA) TT-3′-P |
CA-FAM | 5′-FAM AT TGC TTG CGG CGG TAA CGT CC-3′-P |
CT-TET | 5′-TET CA AAA CGC TTA TTT TGC TAG TGG CC-3′-P |
CP-HEX | 5′-HEX GG TAC AAA CTC CAA AAC TTC TTC CA-3′-P |
CG-FAM | 5′-FAM TA GGT TTT ACC AAC TCG GTG TT GAT-3′-P |
CK-TET | 5′-TET AG TGG CCC GAG CGA ACT AGA CTT TT-3′-P |
Each probe also contained a quencher dye, TAMRA, attached to a linker arm-modified nucleotide near its 3′ end.