TABLE 2.

Detection and identification of Candida species in positive blood cultures with multiple fluorescent probes^a

Blood culture result	Delta RQ values for the following PCRs with the indicated probes:
	PCR A			PCR B		PCR C (All-CAN-TET)
	CA-FAM	CT-TET	CP-HEX	CG-FAM	CK-TET
C. albicans (n = 23)	0.950 ± 0.129	0	0	0	0	3.418 ± 0.140
C. tropicalis (n = 6)	0	0.483 ± 0.156	0	0	0	1.920 ± 0.557
C. parapsilosis (n = 5)	0	0	0.330 ± 0.053	0	0	1.784 ± 0.671
C. glabrata (n = 18)	0	0	0	0.630 ± 0.091	0	2.806 ± 0.291
C. krusei (n = 6)	0	0	0	0	0.727 ± 0.136	3.377 ± 0.378
No growth (n = 10)	0	0	0	0	0	0
Bacteremia (n = 10)	0	0	0	0	0	0

^aValues are expressed as means ± standard errors (see Materials and Methods). Means ± standard error delta RQ values for all negative control samples were as follows: for the CA-FAM probe (n = 55), 0.021 ± 0.003; for the CT-TET probe (n = 72), 0.033 ± 0.006; for the CP-HEX probe (n = 73), 0.052 ± 0.008; for the CG-FAM probe (n = 60), 0.009 ± 0.001; for the CK-TET probe (n = 72), 0.025 ± 0.004; for the All-CAN-TET probe (n = 20), 0.060 ± 0.014. The P value was <0.01 for positive test samples versus negative control samples for all probes.