TABLE 4.
Specificity of the All-CAN-TET fluorescent probe
| Genomic DNA tested | Delta RQ valuesa |
|---|---|
| C. kefyr | 4.843 ± 0.164b |
| C. parapsilosis | 4.587 ± 0.159b |
| C. krusei | 4.560 ± 0.377b |
| C. albicans | 4.361 ± 0.104b |
| C. glabrata | 3.330 ± 0.183b |
| C. tropicalis | 3.273 ± 0.018b |
| S. cerevisiae | 2.270 ± 0.175b |
| C. guilliermondii | 2.180 ± 0.085b |
| A. fumigatus | 1.661 ± 0.180b |
| A. flavus | 1.132 ± 0.181b |
| H. capsulatum | 0.210 ± 0.036 |
| P. aeruginosa | 0.187 ± 0.063 |
| E. coli | 0.177 ± 0.047 |
| C. neoformans | 0.173 ± 0.061 |
| P. marneffei | 0.160 ± 0.051 |
| Human cell line | 0.117 ± 0.093 |
| B. dermatitidis | 0.111 ± 0.110 |
| S. aureus | 0.060 ± 0.046 |
a
Values are means ± standard errors. Data are for triplicate samples from one to three experiments.
b
Positive value based on cutoff of 4 SDs above the mean for negative (no-DNA) control samples (mean ± SD delta RQ value for negative controls [n = 20], 0.060 ± 0.062; 4 SDs above the mean ± 0.308 cutoff for positivity).