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. 2021 Aug 2;22(9):e52911. doi: 10.15252/embr.202152911

Figure EV4. Modeling of the docking complex.

Figure EV4

  • A
    The DC density on the doublet microtubule between PFs‐A7 and A8.
  • B
    Prediction of coiled coil for DC1 and DC2 using COILS with a window size of 28 (Lupas et al, 1991).
  • C
    Sequence alignment of Chlamydomonas DC1 and Tetrahymena CCDC151 homolog (Q22T00).
  • D–G
    Structures of Chlamydomonas ODA reconstituted on microtubules with biotin carboxyl carrier protein (BCCP) tagged in different regions of DC2 (residue 76, 276, 412, and 507) from Oda et al (2016a) (EMD‐6508, 6509, 6510, 6511). The enhanced signals of BCCP‐tag are indicated in colors.
  • H
    Slice from a density map showing the docking complex (position indicated in the cartoon). The yellow line indicates one continuous DC1/2.
  • I
    The globular density at the end of the coiled coil of DC1/2 (black arrow and dotted line).
  • J
    The model of the DC based on our analysis.