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. 2021 Jul 29;22(9):e51328. doi: 10.15252/embr.202051328

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© 2021 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure EV2 — BioID2‐tagged endophilin A2 construct and assay workflow.

Proteins significantly enriched in biotinylated fraction of endophilin A2‐BioID2 samples compared with empty plasmid control (positive protein enrichment scores). Negative enrichment score represents gene products enriched in BioID2 control sample, reflecting the promiscuous nature of the cytoplasmic biotinylase. Data from 2 independent experiments, see also Dataset EV4.