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. 2021 Jul 29;22(9):e51328. doi: 10.15252/embr.202051328

Figure 3. Endophilin A2 is required for peripheral B‐cell development and GC responses.

Figure 3

  1. CRISPR‐targeted bone marrow chimera workflow, showing flow cytometry detection of donor‐derived (GFP+), sgRNA‐containing (mCherry+) cells.
  2. Development of B‐cell populations in chimeras' bone marrow and spleen from Cd4‐ or Sh3gl1‐targeted HSCs. Percentage of mCherry+ cells at each stage is normalized to initial infection rate of transferred lineage‐ cells, and shown as mean + SEM. N = 11–12 mice. *P = 0.0286, ***P < 0.0001 using two‐way ANOVA.
  3. MZ development in mCherry+ B‐cell compartment.
  4. Representative stain of surface CD86 and CD69 on adoptively transferred HEL‐specific follicular B cells 24 h post‐SRBC‐HEL immunization.
  5. Quantification of surface CD86 and CD69 mean fluorescence intensity (MFI). Data show mean and SEM from six mice across two experiments.
  6. Fractions of mCherry+ cells in GC, PC, IgG1+ class‐switched and follicular B cells at 4‐, 8‐, and 14 days post‐SRBC‐HEL immunization. N = 8–10 mice; data shows mean and SEM. *P < 0.05; ***P < 0.001; ****P < 0.0001 using 2‐way ANOVA with multiple comparisons.