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. 2021 Aug 11;20(9):4427–4434. doi: 10.1021/acs.jproteome.1c00349

Figure 1.

Figure 1

Intact Mass Analysis of COVID RBD. (A) Optimized Mt. Sinai construct (M67) and (B) optimized Ragon construct (M68) following PNGase F treatment. The insets correspond to the ReSpect deconvolution of the respective MS1 scans, with significant species labeled. Both constructs had abundant O-glycans of Hex(1)HexNAc(1)NeuAc(2) and Hex(2)HexNAc(2)NeuAc(2). The observed O-glycan masses in the M67 construct had an additional unknown mass of 111 Da. The M68 construct had an unknown mass shift of 87 Da from each abundant O-glycan and a mass shift of 158 Da from the first unknown shift, for a total of 245 Da from the unmodified protein.