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. 2021 Jul 28;20(9):4610–4620. doi: 10.1021/acs.jproteome.1c00378

Table 1. Summary of Number of Identified Proteins, Peptides, and PSM.

  plasma depletion procedure
 LCM5/MS
experiment commercial products company loading capacity number of use(s) capturing agents ideally captured proteins operation labeling HiRIEF pH depleted plasma (mg) # PSM # peptides # proteins
Set 1a High Select ThermoFisher Scientific Up to 10 μL (load 10 μL) Single Antibodies Albumin Alpha-1-acidglycoprotein Centrifugec TMTpro 16-plex 3–10 0.72e 101 725 16347 1913
  Top14         IgA Alpha-1-antitypsin              
  Abundant         IgD Alpha-2-macroglobulin              
  Protein         IgE Apolipoprotein a A1              
  Depletion         IgG Fibrinogen              
  Mini Spin         IgG (light chain) Haptogloblin              
  Columns         IgM Transferrin              
Set 2a High Select ThermoFisher Scientific Up to 10 μL (load 10 μL) Single Antibodies Albumin Alpha-1-acidglycoprotein Centrifugec TMTpro 16-plex 3–10 1.04e 119 395 16449 1897
  Top14         IgA Alpha-1-antitypsin              
  Abundant         IgD Alpha-2-macroglobulin              
  Protein         IgE Apolipoprotein a A1              
  Depletion         IgG Fibrinogen              
  Mini Spin         IgG (light chain) Haptogloblin              
  Columns         IgM Transferrin              
Set 3a Multiply Agilent 30–70 μL (load 10 μL) Multiple (>200 Injections) Antibodies Albumin Alpha-1-acid glycoprotein HPLCd TMTpro 16-plex 3–10 0.96e 109 157 16360 1878
  Affinity         IgA Antitypsin              
  Removal         IgG Alpha-2-macroglobulin              
  Column         IgM Apolipoprotein a A1              
  Human 14         Fibrinogen Apolipoprotein a A2              
            Haptogloblin Complement C3              
            Transferrin Transthyretin              
Set 4b High Select ThermoFisher Scientific Up to 10 μL (load 10 μL) Single Antibodies Albumin Alpha-1-acid glycoprotein Centrifugec TMTpro 10-plex 3–10 0.48e 64900 12854 2039
  Top14         IgA Alpha-1-antitypsin              
  Abundant         IgD Alpha-2-macroglobulin              
  Protein         IgE Apolipoprotein a A1              
  Depletion         IgG Fibrinogen              
  Mini Spin         IgG (light chain) Haptogloblin              
  Columns         IgM Transferrin              
a

The same 14 human plasma samples plus duplicates of the internal standard.

b

The same individual female plasma sample with PSA: w/o heating ×4, depletion ×3, heating after depletion ×3.

c

Avg. 40 min per 14 samples.

d

40 min per sample, plus buffer wash (40 min) between two samples.

e

Load half to LC-MS/MS.