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. 2021 Aug 23;20(9):4507–4517. doi: 10.1021/acs.jproteome.1c00463

Figure 6.

Figure 6

Peptidomics of secretions from mouse islets. (a–c) Peak areas of peptides in supernatants of islets cultured in low, medium, or high glucose, or low glucose + adrenaline. IAPP and insulin-derived peptides (a), proglucagon-derived peptides (b), PPY and SST-14 (c). Individual data points are displayed in each plot along with the mean. Conditions were as follows; LG = 1 mmol/L glucose, LG + Adr = 1 mmol/L glucose + 5 μmol/L adrenaline, MG = 6 mmol/L glucose, HG = 16.7 mmol/L glucose. n = 5 with each data point representing pooled supernatants from 9 tubes each containing 15 islets. Islets obtained from 15 mice. (d) Heatmap of mouse islet secretion peptidomics. Data presented as Z-score of each row. Gray squares within the heatmap indicate where peptides were not detectable. PyySP_68-100 is a peptide from a splice variant of Pyy which has 2 amino acids inserted into the C-terminus of the propeptide. It was quantified in lieu of Pyy_68-98 which was undetectable.