Table 2.
Changes in gene expression in adult microglia exposed to TLR agonists for 10 h relative to unexposed adult microglia in medium alone
| Gene | LPS (n = 3) | IMQ (n = 3) | CpG-ODN 1585 (n = 3) | |||
|---|---|---|---|---|---|---|
| FC | P value | FC | P value | FC | P value | |
| Ccl1 | − 1.7 | 3.0E-01 | − 4.6 | 7.3E-03 | − 4.4 | 1.6E-02 |
| Ccl11 | − 1.2 | 8.5E-01 | − 5.3 | 1.8E-02 | − 5.2 | 3.1E-02 |
| Ccl12 | 13.0 | 4.5E-03 | − 2.0 | 2.4E-01 | 1.2 | 7.6E-01 |
| Ccl17 | 76.1 | 6.8E-03 | 25.4 | 9.6E-04 | 4.7 | 3.6E-02 |
| Ccl2 | 85.9 | 1.4E-04 | 2.6 | 7.3E-03 | − 2.0 | 2.2E-01 |
| Ccl20 | 330.2 | 3.5E-05 | 15.7 | 2.0E-03 | − 3.3 | 2.1E-02 |
| Ccl22 | 7.8 | 6.6E-02 | 1.6 | 6.3E-01 | 1.3 | 8.0E-01 |
| Ccl24 | − 2.0 | 1.9E-01 | − 4.2 | 1.2E-02 | − 3.6 | 2.9E-02 |
| Ccl3 | 155.5 | 3.9E-04 | 22.2 | 4.1E-03 | − 1.5 | 2.9E-01 |
| Ccl4 | 48.8 | 1.7E-03 | 4.4 | 4.7E-02 | − 1.6 | 2.7E-01 |
| Ccl5 | 1671.6 | 6.9E-05 | 127.7 | 2.9E-04 | − 1.3 | 7.9E-01 |
| Ccl7 | 58.0 | 9.0E-04 | 2.7 | 1.7E-01 | − 1.0 | 9.8E-01 |
| Ccl8 | 4.3 | 1.2E-01 | -1.2 | 8.1E-01 | − 3.3 | 2.1E-02 |
| Ccl9 | 24.3 | 1.2E-02 | 13.7 | 1.9E-02 | − 1.1 | 8.8E-01 |
| Ccr1 | 2.7 | 2.3E-02 | 1.6 | 2.6E-01 | 1.4 | 4.9E-01 |
| Ccr10 | − 2.3 | 1.0E-04 | − 3.5 | 7.3E-03 | − 3.3 | 2.1E-02 |
| Ccr2 | − 1.3 | 7.1E-01 | − 1.6 | 5.7E-01 | − 3.3 | 2.1E-02 |
| Ccr4 | − 2.0 | 3.6E-01 | − 5.1 | 2.9E-02 | − 3.3 | 7.9E-02 |
| Ccr5 | − 2.5 | 9.1E-02 | − 4.5 | 7.1E-03 | 1.2 | 5.9E-01 |
| Ccr6 | − 1.2 | 8.3E-01 | − 3.8 | 7.1E-03 | − 3.2 | 1.2E-02 |
| Ccr8 | − 2.3 | 1.0E-04 | − 3.5 | 7.3E-03 | − 3.3 | 2.1E-02 |
| Cd40lg | − 2.7 | 2.0E-03 | − 3.9 | 7.7E-03 | − 3.8 | 1.9E-02 |
| Csf2 | 211.5 | 1.1E-03 | 8.9 | 6.8E-02 | − 1.0 | 9.7E-01 |
| Csf3 | 13,079.3 | 3.0E-05 | 553.8 | 1.8E-06 | − 1.8 | 4.2E-01 |
| Cxcl1 | 1,668.9 | 9.1E-06 | 160.9 | 1.2E-05 | − 1.9 | 2.2E-01 |
| Cxcl10 | 2,193.5 | 7.7E-05 | 10.7 | 3.4E-02 | − 19.3 | 3.6E-02 |
| Cxcl13 | − 2.9 | 9.8E-03 | − 4.4 | 1.2E-02 | − 4.2 | 2.3E-02 |
| Cxcl15 | − 2.4 | 1.7E-01 | − 6.1 | 3.3E-02 | − 6.9 | 1.9E-02 |
| Cxcl5 | 30.3 | 4.7E-05 | 2.1 | 1.4E-01 | 1.1 | 9.1E-01 |
| Cxcl9 | − 2.1 | 4.9E-03 | − 3.5 | 7.3E-03 | − 3.3 | 2.1E-02 |
| Cxcr2 | − 3.6 | 9.8E-02 | − 6.0 | 4.8E-02 | − 2.1 | 5.4E-01 |
| Cxcr5 | 1.0 | 9.8E-01 | − 9.6 | 2.1E-02 | − 4.3 | 1.8E-01 |
| Fasl | − 2.3 | 1.0E-04 | − 3.5 | 7.3E-03 | − 3.3 | 2.1E-02 |
| Ifng | 11.0 | 4.0E-02 | 1.2 | 8.7E-01 | − 6.1 | 6.7E-02 |
| Il11 | 7.2 | 3.3E-02 | 1.8 | 6.1E-01 | − 1.7 | 6.5E-01 |
| Il13 | 31.4 | 6.4E-04 | − 1.3 | 8.2E-01 | − 3.3 | 2.1E-02 |
| Il15 | 16.1 | 3.1E-03 | − 2.1 | 9.7E-02 | − 1.8 | 1.5E-01 |
| Il17a | − 2.2 | 1.8E-03 | − 3.5 | 7.3E-03 | − 3.4 | 2.1E-02 |
| Il17b | − 1.8 | 7.1E-01 | − 5.9 | 1.3E-01 | − 9.6 | 3.0E-02 |
| Il17f | − 1.5 | 1.4E-01 | 1.2 | 8.9E-01 | − 3.3 | 1.9E-02 |
| Il1a | 595.2 | 2.9E-05 | 34.2 | 7.2E-05 | − 1.4 | 3.9E-01 |
| Il1b | 5258.1 | 1.9E-05 | 248.9 | 1.1E-04 | − 1.3 | 7.1E-01 |
| Il1rn | 52.8 | 9.3E-04 | 6.3 | 1.4E-02 | − 1.5 | 2.7E-01 |
| Il21 | − 4.0 | 5.0E-02 | − 6.0 | 3.3E-02 | − 5.7 | 4.3E-02 |
| Il27 | 1463.6 | 4.7E-06 | 5.8 | 3.6E-02 | 1.3 | 8.6E-01 |
| Il2rb | − 1.4 | 6.2E-01 | − 3.5 | 7.3E-03 | − 3.3 | 2.1E-02 |
| Il33 | 2.0 | 4.3E-01 | − 3.5 | 7.3E-03 | − 3.3 | 2.1E-02 |
| Il4 | − 1.7 | 1.8E-01 | − 1.6 | 3.5E-01 | − 3.3 | 2.1E-02 |
| Il5 | − 2.0 | 5.2E-01 | − 6.7 | 5.4E-02 | − 10.6 | 2.9E-02 |
| Il5ra | − 2.2 | 2.5E-04 | − 3.5 | 7.3E-03 | − 1.8 | 1.6E-03 |
| Nampt | 4.6 | 1.1E-01 | 1.2 | 8.3E-01 | − 4.8 | 2.7E-02 |
| Spp1 | − 3.7 | 3.6E-02 | − 2.6 | 3.6E-01 | − 3.2 | 1.1E-01 |
| Tnf | 3.5 | 5.5E-04 | 1.7 | 2.5E-02 | − 1.0 | 6.7E-01 |
| Tnfsf11 | − 2.2 | 1.8E-02 | − 3.1 | 1.0E-03 | − 1.3 | 1.0E-01 |
| Tnfsf4 | 89.9 | 1.0E-03 | 19.0 | 2.7E-03 | 1.5 | 4.0E-01 |
n is the number of independent microglia RNA samples tested. Fold change (FC) relative to unexposed adult microglia. P values are calculated using the student t-test on the Delta CT. Bolded values reached our criteria of statistical significance where the FC is greater or less than 2-fold with a P value ≤ 5.0E-02