Table 3.
Top 20 proinflammatory genes significantly increased in the brain of untreated mice after 6 h of exposure to TLR agonists relative to PBS-treated control mice (n = 6)
| LPS (n = 7) | IMQ (n = 7) | CpG-ODN 1585 (n = 6) | ||||||
|---|---|---|---|---|---|---|---|---|
| Gene | FC | P value | Gene | FC | P value | Gene | FC | P value |
| Cxcl10 | 545.7 | 2.9E-09 | Cxcl10 | 203.4 | 4.0E-10 | Cxcl10 | 648.2 | < 1.0E-10 |
| Ccl20 | 189.7 | 3.3E-07 | Ccl20 | 39.4 | 1.9E-04 | Ccl20 | 383.3 | < 1.0E-10 |
| Il1rn | 157.3 | 6.0E-07 | Ccl2 | 33.0 | 7.0E-05 | Il1rn | 115.6 | 4.0E-07 |
| Cxcl1 | 95.2 | 4.9E-06 | Ccl7 | 29.1 | 1.9E-05 | Ccl2 | 103.3 | 4.5E-07 |
| Ccl2 | 91.2 | 1.4E-05 | Il1rn | 25.1 | 1.9E-05 | Ccl7 | 68.2 | 3.6E-07 |
| Ccl5 | 82.7 | 7.7E-08 | Ccl4 | 19.7 | 4.8E-05 | Il1b | 66.1 | 4.3E-06 |
| Csf3 | 79.9 | 2.2E-08 | Ccl3 | 18.9 | 1.4E-07 | Ccl4 | 64.4 | 1.8E-06 |
| Ccl3 | 64.6 | 5.5E-08 | Ccl12 | 16.8 | 1.1E-05 | Ccl3 | 59.3 | 8.2E-09 |
| Il1b | 58.1 | 4.3E-06 | Tnf | 9.6 | 5.5E-05 | Tnf | 52.1 | 1.6E-07 |
| Ccl4 | 55.4 | 5.8E-06 | Ccl11 | 9.6 | 6.4E-04 | Csf3 | 47.0 | 5.8E-08 |
| Ccl7 | 52.5 | 6.3E-05 | Cxcl11 | 9.1 | 2.7E-03 | Cxcl1 | 46.5 | 2.9E-06 |
| Ccl11 | 43.0 | 2.7E-05 | Cxcl13 | 8.5 | 3.6E-04 | Ccl5 | 41.5 | 9.0E-10 |
| Cxcl5 | 27.7 | 7.9E-04 | Ccl5 | 7.8 | 2.6E-06 | Ccl11 | 26.1 | 2.1E-05 |
| Il1a | 25.3 | 1.5E-06 | Csf3 | 7.6 | 1.0E-03 | Ccl12 | 21.0 | 4.4E-07 |
| Tnf | 23.8 | 5.8E-04 | Cxcl1 | 6.8 | 1.7E-02 | Cxcl5 | 20.8 | 8.1E-05 |
| Cxcl9 | 17.7 | 8.2E-05 | Il1b | 6.7 | 1.6E-02 | Cxcl11 | 18.9 | 1.7E-05 |
| Ccl22 | 16.7 | 1.6E-05 | Cxcl5 | 6.1 | 2.8E-02 | Cxcr2 | 16.9 | 1.5E-04 |
| Ccl12 | 16.4 | 1.7E-04 | Tnfsf10 | 5.2 | 1.8E-06 | Cxcl9 | 10.8 | 1.6E-08 |
| Ccl9 | 15.5 | 7.9E-04 | Il1a | 3.9 | 8.3E-04 | Il1a | 10.1 | 7.5E-08 |
| Tnfsf11 | 9.0 | 1.2E-03 | Ccr5 | 3.6 | 1.2E-02 | Tnfsf10 | 9.5 | 4.4E-08 |
n is the number of independent mouse RNA samples tested. Fold change (FC) relative to PBS-treated mice. P values are calculated using the student t-test on the Delta CT. All changes reached our criteria of statistical significance where the FC is greater than 2-fold with a P value ≤ 5.0E-02