Table 4.
Proinflammatory genes significantly decreased in the brain of PLX5622-treated relative to untreated mice after 6 h of exposure to TLR agonists
| PLX5622-treated (n = 7) relative to Untreated exposed to LPS (n = 7) | PLX5622-treated (n = 7) relative to Untreated exposed to IMQ (n = 7) | PLX5622-treated (n = 6) relative to Untreated exposed to CpG-ODN 1585 (n = 6) | ||||||
|---|---|---|---|---|---|---|---|---|
| Gene | FC | P value | Gene | FC | P value | Gene | FC | P value |
| Ccl20 | − 11.1 | 5.1E-03 | Cxcl10 | − 18.6 | 9.9E-05 | Ccl20 | − 160.8 | 4.4E-06 |
| Ccl9 | − 8.8 | 6.8E-05 | Ccl3 | − 15.1 | 4.4E-06 | Ccl3 | − 14.1 | 7.5E-07 |
| Ccl12 | − 7.4 | 3.1E-04 | Tnf | − 14.2 | 2.9E-04 | Ccl8 | − 9.4 | 7.4E-05 |
| Il1a | − 6.5 | 3.7E-04 | Ccl20 | − 12.9 | 4.8E-03 | Ccl4 | − 9.1 | 1.1E-06 |
| Pf4 | − 6.2 | 8.9E-06 | Ccl4 | − 10.4 | 2.6E-06 | Ccr5 | − 9.0 | 8.1E-05 |
| Il1rn | − 5.1 | 3.7E-04 | Ccl8 | − 9.6 | 5.0E-04 | Il1a | − 8.1 | 4.9E-06 |
| Ccr3 | − 5.1 | 2.1E-03 | Cxcl11 | − 9.4 | 5.6E-04 | Ccl12 | − 8.0 | 3.9E-06 |
| Ccl3 | − 5.1 | 3.2E-04 | Pf4 | − 9.2 | 5.0E-06 | Cxcl5 | − 7.7 | 1.8E-05 |
| Ccl4 | − 5.1 | 7.5E-04 | Ccl12 | − 9.0 | 5.1E-05 | Ccl9 | − 6.9 | 1.4E-04 |
| Ccl8 | − 5.0 | 1.2E-02 | Ccl2 | − 8.1 | 1.3E-03 | Tnf | − 6.8 | 7.1E-06 |
| Ccr5 | − 4.9 | 1.0E-04 | Il1rn | − 7.6 | 6.0E-05 | Pf4 | − 6.5 | 7.3E-06 |
| Il27 | − 3.2 | 3.3E-02 | Ccl7 | − 6.9 | 2.9E-04 | Ccr3 | − 6.2 | 3.6E-04 |
| Ccl5 | − 3.0 | 6.1E-03 | Cxcl13 | − 6.0 | 5.5E-03 | Csf3 | − 6.1 | 3.4E-07 |
| Il1b | − 2.6 | 3.2E-02 | Ccr5 | − 5.3 | 3.3E-05 | Il1rn | − 6.0 | 1.2E-05 |
| Ifng | − 2.2 | 4.5E-02 | Ccl9 | − 4.8 | 2.4E-03 | Ccl5 | − 5.5 | 1.3E-06 |
| Tnfsf10 | − 2.1 | 1.5E-02 | Il1b | − 4.7 | 2.4E-02 | Cxcl1 | − 5.4 | 5.9E-04 |
| Ccl6 | − 4.7 | 1.4E-04 | Tnfsf11 | − 5.2 | 3.7E-03 | |||
| Ccl5 | − 4.4 | 8.6E-05 | Ccl6 | − 5.2 | 1.8E-04 | |||
| Il1a | − 4.0 | 5.8E-03 | Ccr1 | − 4.5 | 1.8E-04 | |||
| Ccr2 | − 4.0 | 2.0E-02 | Cxcr2 | − 4.4 | 2.5E-03 | |||
| Cxcl1 | − 3.9 | 5.1E-02 | Il27 | − 4.3 | 1.2E-02 | |||
| Csf3 | − 3.6 | 9.0E-03 | Cxcl11 | − 4.1 | 3.2E-04 | |||
| Ccr1 | − 3.5 | 6.8E-03 | Ccl22 | − 3.4 | 1.6E-06 | |||
| Il27 | − 3.4 | 5.4E-03 | Ccl11 | − 3.3 | 2.6E-05 | |||
| Ccr3 | − 3.4 | 1.6E-04 | Cxcl10 | − 3.3 | 1.7E-06 | |||
| Osm | − 3.3 | 1.2E-02 | Cxcl9 | − 3.3 | 2.8E-02 | |||
| Tnfsf10 | − 3.2 | 2.9E-03 | Il2rg | − 2.9 | 3.9E-05 | |||
| Ccl11 | − 3.2 | 8.8E-04 | Il1b | − 2.8 | 8.7E-03 | |||
| Cxcl9 | − 2.7 | 3.7E-02 | Osm | − 2.7 | 1.1E-02 | |||
| Il10ra | − 2.3 | 1.9E-03 | Tnfsf10 | − 2.6 | 8.7E-06 | |||
| Ccl7 | − 2.4 | 5.7E-05 | ||||||
| Il6ra | − 2.2 | 5.6E-04 | ||||||
| Il10ra | − 2.1 | 2.8E-03 | ||||||
| Ccl2 | − 2.0 | 1.3E-03 | ||||||
n is the number of independent mouse RNA samples tested. FC is fold change in gene expression as indicated. P values are calculated using the student t test on the Delta CT. All changes reached our criteria of statistical significance where the FC is less than 2.0-fold with a P value ≤ 5.0E-02