Table 5.
TLR signaling pathway associated genes significantly altered in the brain of untreated mice after 6 h of exposure to TLR agonists relative to PBS-treated control mice (n = 3)
| LPS (n = 4) | IMQ (n = 4) | CpG-ODN 1585 (n = 3) | ||||||
|---|---|---|---|---|---|---|---|---|
| Gene | FC | P value | Gene | FC | P value | Gene | FC | P value |
| Cxcl10 | 500.5 | < 1.0E-10 | Cxcl10 | 182.7 | < 1.0E-10 | Cxcl10 | 873.3 | < 1.0E-10 |
| Ccl2 | 110.3 | < 1.0E-10 | Ccl2 | 28.6 | < 1.0E-10 | Ccl2 | 182.9 | < 1.0E-10 |
| Il6 | 65.8 | < 1.0E-10 | Tnf | 14.0 | < 1.0E-10 | Il6 | 108.0 | < 1.0E-10 |
| Csf3 | 51.2 | < 1.0E-10 | Il6 | 10.8 | < 1.0E-10 | Tnf | 72.9 | < 1.0E-10 |
| Il1b | 49.5 | < 1.0E-10 | Tlr2 | 4.2 | 2.0E-07 | Il1b | 69.7 | < 1.0E-10 |
| Tnf | 42.0 | < 1.0E-10 | Il1b | 3.8 | 1.1E-06 | Clec4e | 68.2 | < 1.0E-10 |
| Clec4e | 29.0 | < 1.0E-10 | Eif2ak2 | 3.3 | 1.4E-05 | Csf3 | 32.5 | < 1.0E-10 |
| Il1a | 17.1 | < 1.0E-10 | Irf1 | 2.6 | 4.0E-04 | Tlr2 | 21.0 | < 1.0E-10 |
| Tlr2 | 16.4 | < 1.0E-10 | Csf3 | 2.6 | 4.8E-04 | Cd14 | 12.6 | < 1.0E-10 |
| Cd14 | 11.9 | < 1.0E-10 | Tlr1 | 2.5 | 6.4E-04 | Il1a | 10.6 | < 1.0E-10 |
| Tlr1 | 7.1 | < 1.0E-10 | Il1a | 2.2 | 3.3E-03 | Il10 | 7.6 | 2.1E-09 |
| Il10 | 6.8 | < 1.0E-10 | Myd88 | 2.1 | 5.8E-03 | Tlr1 | 6.9 | 1.1E-08 |
| Nfkbia | 5.5 | 1.5E-09 | Cd14 | 2.1 | 5.8E-03 | Eif2ak2 | 6.0 | 9.1E-08 |
| Eif2ak2 | 4.3 | 2.1E-07 | Ifnb1 | − 4.4 | 7.9E-08 | Irf1 | 5.8 | 1.7E-07 |
| Myd88 | 4.1 | 4.6E-07 | Csf2 | − 82.5 | < 1.0E-10 | Ifnb1 | 5.5 | 3.7E-07 |
| Tnfaip3 | 4.0 | 8.6E-07 | Myd88 | 4.9 | 1.9E-06 | |||
| Irf1 | 3.8 | 1.4E-06 | Nfkbia | 4.4 | 8.0E-06 | |||
| Cd86 | 3.7 | 2.3E-06 | Tnfaip3 | 3.7 | 8.2E-05 | |||
| Cd80 | 3.1 | 3.8E-05 | Tlr3 | 3.0 | 8.9E-04 | |||
| Nfkb2 | 2.7 | 3.1E-04 | Tlr8 | 3.0 | 9.6E-04 | |||
| Ptgs2 | 2.7 | 3.1E-04 | Cd80 | 2.8 | 2.0E-03 | |||
| Ifng | 2.6 | 7.2E-04 | Nfkb2 | 2.8 | 2.1E-03 | |||
| Tnfrsf1a | 2.4 | 1.5E-03 | Cd86 | 2.6 | 4.7E-03 | |||
| Ripk2 | 2.3 | 2.0E-03 | Ripk2 | 2.5 | 5.7E-03 | |||
| Cebpb | 2.3 | 2.8E-03 | Casp8 | 2.5 | 5.7E-03 | |||
| Il1r1 | 2.1 | 7.2E-03 | Tnfrsf1a | 2.5 | 6.4E-03 | |||
| Tlr3 | 2.1 | 6.7E-03 | Ptgs2 | 2.4 | 7.3E-03 | |||
| Pglyrp1 | 2.0 | 9.7E-03 | Tlr6 | 2.3 | 1.0E-02 | |||
| Fos | − 2.0 | 9.7E-03 | Muc13 | 2.0 | 3.1E-02 | |||
| Ifnb1 | − 3.1 | 4.7E-05 | Nfkbil1 | − 2.1 | 2.5E-02 | |||
| Csf2 | − 49.4 | < 1.0E-10 | Csf2 | − 43.7 | < 1.0E-10 | |||
n is the number of independent mouse RNA samples tested. Fold change (FC) relative to PBS treated mice. P values are calculated using the student t-test on the Delta CT. All changes reached our criteria of statistical significance where the FC is greater or less than 2-fold with a P value ≤ 5.0E-02