Table 6.
TLR signaling pathway associated genes altered in the brain of PLX5622-treated relative to Untreated mice after 6 hours of exposure to TLR agonists or PBS
| PLX5622-treated (n = 3) relative to Untreated exposed to PBS (n = 3) | PLX5622-treated (n = 4) relative to Untreated exposed to LPS (n = 4) | PLX5622-treated (n = 4) relative to Untreated exposed to IMQ (n = 4) | PLX5622-treated (n = 3) relative to Untreated exposed to CpG-ODN 1585 (n = 3) | |||||
|---|---|---|---|---|---|---|---|---|
| Gene | FC | P value | FC | P value | FC | P value | FC | P value |
| Btk | − 2.2 | 8.5E-03 | − 4.2 | 5.2E-07 | − 3.0 | 2.8E-04 | − 2.3 | 1.1E-02 |
| Casp8 | − 1.3 | 3.5E-01 | − 1.8 | 4.6E-02 | − 2.0 | 2.0E-02 | − 2.9 | 1.0E-03 |
| Ccl2 | − 1.4 | 2.6E-01 | − 1.0 | 9.8E-01 | − 8.0 | < 1.1E-10 | − 1.6 | 1.6E-01 |
| Cd14 | − 2.6 | 1.4E-03 | − 3.0 | 1.2E-04 | − 3.9 | 8.9E-06 | − 4.1 | 1.6E-05 |
| Cd80 | − 1.5 | 2.1E-01 | − 1.4 | 2.4E-01 | − 1.4 | 2.6E-01 | − 2.2 | 1.9E-02 |
| Cd86 | − 6.1 | 4.9E-09 | − 13.6 | < 1.1E-10 | − 5.1 | 9.7E-08 | − 11.0 | < 1.1E-10 |
| Clec4e | 1.3 | 4.0E-01 | − 20.5 | < 1.1E-10 | − 1.3 | 4.2E-01 | − 3.4 | 1.6E-04 |
| Csf2 | − 1.7 | 6.6E-02 | 2.3 | 4.4E-03 | 2.2 | 1.0E-02 | − 1.5 | 2.5E-01 |
| Csf3 | 1.2 | 5.3E-01 | 1.6 | 8.3E-02 | − 2.4 | 3.9E-03 | − 3.9 | 3.4E-05 |
| Cxcl10 | 1.8 | 6.0E-02 | − 1.4 | 2.8E-01 | − 13.2 | < 1.1E-10 | − 2.6 | 3.4E-03 |
| Ifnb1 | 1.2 | 5.0E-01 | 1.2 | 5.3E-01 | 1.3 | 3.6E-01 | − 2.2 | 1.4E-02 |
| Ifng | 2.5 | 3.0E-03 | 1.2 | 5.6E-01 | 1.0 | 9.8E-01 | 4.4 | 6.8E-06 |
| Il10 | 1.2 | 4.8E-01 | − 4.0 | 1.5E-06 | 1.1 | 8.7E-01 | − 3.2 | 4.3E-04 |
| Il1a | − 1.5 | 2.1E-01 | − 5.8 | 1.1E-09 | − 2.9 | 5.1E-04 | − 7.0 | 4.3E-09 |
| Il1b | − 1.1 | 6.6E-01 | − 4.1 | 7.5E-07 | − 2.9 | 5.1E-04 | − 2.1 | 2.0E-02 |
| Il6 | − 1.6 | 1.2E-01 | − 1.6 | 8.8E-02 | − 5.2 | 7.6E-08 | − 2.0 | 3.2E-02 |
| Irf1 | 1.3 | 4.2E-01 | 1.2 | 4.9E-01 | − 2.0 | 2.4E-02 | − 1.6 | 1.3E-01 |
| Ly86 | − 3.5 | 4.8E-05 | − 10.7 | < 1.1E-10 | − 3.2 | 1.5E-04 | − 4.3 | 1.0E-05 |
| Nfkbib | 1.2 | 6.5E-01 | − 2.0 | 1.6E-02 | − 1.1 | 8.2E-01 | 1.5 | 1.9E-01 |
| Ticam2 | − 3.8 | 1.2E-05 | − 8.7 | < 1.1E-10 | − 5.5 | 2.5E-08 | − 2.7 | 2.7E-03 |
| Tlr1 | − 7.4 | 1.0E-10 | − 28.1 | < 1.1E-10 | − 7.3 | < 1.1E-10 | − 12.0 | < 1.1E-10 |
| Tlr2 | 1.2 | 6.3E-01 | − 2.1 | 8.6E-03 | − 3.4 | 5.3E-05 | − 3.2 | 4.0E-04 |
| Tlr5 | − 1.3 | 4.5E-01 | − 1.3 | 4.2E-01 | − 2.9 | 4.3E-04 | − 1.8 | 6.7E-02 |
| Tlr6 | − 3.4 | 5.8E-05 | − 5.2 | 1.1E-08 | − 1.7 | 9.8E-02 | − 5.4 | 3.5E-07 |
| Tlr7 | − 4.3 | 2.1E-06 | − 17.1 | < 1.1E-10 | − 5.6 | 1.7E-08 | − 6.8 | 6.2E-09 |
| Tlr8 | − 4.6 | 7.6E-07 | − 13.5 | < 1.1E-10 | − 12.6 | < 1.1E-10 | − 1.7 | 1.2E-01 |
| Tlr9 | − 1.8 | 6.3E-02 | − 1.2 | 4.8E-01 | − 2.6 | 1.7E-03 | − 1.4 | 2.6E-01 |
| Tnf | 1.6 | 1.1E-01 | − 10.1 | < 1.1E-10 | − 11.4 | < 1.1E-10 | − 4.5 | 4.6E-06 |
n is the number of independent mouse RNA samples tested. FC is fold change in gene expression as indicated. P values are calculated using the student t-test on the Delta CT. All changes in bold reached our criteria of statistical significance where FC is greater or less than 2.0-fold with a P value ≤ 5.0E-02