Fig. 6.

Acid resistance of stationary-phase cultures of Levilactobacillus brevis Lbr-6108 and Lbr-35. The survival of the strains was determined by overnight culture in MRS followed by acid challenge at pH 3.0 in a basal medium solution and pH 3.0 in 0.5% saline solution. All cultures were grown to stationary phase overnight at 37 °C. Viable cell counts were assessed immediately, and recovery rates were determined at 2 h and 3 h following exposure to acidic environment. Values represent the mean of four biological replicates plated in quadruplicate, and error bars represent the standard error. Bars with **** differ significantly (p < 0.0001) according to two-way ANOVA with Tukey’s multiple comparisons test