Table 1.
Top twenty highest expressed genes in Levilactobacillus brevis Lbr-6108 (top) and Lbr-35 (bottom) comparing T18 to T6 in MRS with 10,000 μg/mL monosodium glutamate
| Genes | Lbr-6108 | Lbr-35 | ||||
|---|---|---|---|---|---|---|
| log2 fold change | Adjusted p-value | Reads base mean | log2 fold change | Adjusted p-value | Reads base mean | |
| Uronate isomerase (EC 5.3.1.12) | 4.90 | 4.71E−139 | 10,468 | 2.29 | 4.39E−29 | 1311 |
| NrdR-regulated deoxyribonucleotide transporter, PnuC-like | 4.67 | 5.67E−148 | 2208 | 3.22 | 6.92E−51 | 1278 |
| Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1) | 4.41 | 1.19E−258 | 79,074 | 2.86 | 1.08E−108 | 52,006 |
| Glucuronide transporter UidB | 4.31 | 5.52E−112 | 5426 | 1.56 | 1.54E−19 | 477 |
| beta-glucuronidase (EC 3.2.1.31) | 4.30 | 2.64E−165 | 2277 | 1.69 | 2.55E−16 | 491 |
| Alpha-glucosidase (EC 3.2.1.20) | 4.10 | 4.32E−152 | 7925 | 1.72 | 1.95E−25 | 1533 |
| Galactokinase (EC 2.7.1.6) | 4.01 | 1.11E−47 | 1609 | 1.05 | 5.53E−06 | 299 |
| Lactose and galactose permease, GPH translocator family | 3.99 | 2.62E−55 | 1442 | 0.39 | 5.80E−02 | 362 |
| hypothetical protein | 3.97 | 6.30E−32 | 152 | 0.95 | 1.90E−03 | 65 |
| Tyrosyl-tRNA synthetase (EC 6.1.1.1) | 3.86 | 1.31E−243 | 10,243 | 0.58 | 9.50E−07 | 2184 |
| Carbamate kinase (EC 2.7.2.2) | 3.86 | 7.94E−201 | 7896 | 1.70 | 1.30E−23 | 1,713 |
| Probable glutamate/gamma-aminobutyrate antiporter | 3.79 | 2.11E−164 | 20,971 | − 0.12 | 5.07E−01 | 2237 |
| Glutamate decarboxylase GadA (EC 4.1.1.15) | 3.74 | 7.24E−173 | 26,711 | 0.17 | 2.39E−01 | 2969 |
| Phosphopentomutase (EC 5.4.2.7) | 3.64 | 2.41E−66 | 6719 | − 0.72 | 4.59E−04 | 2996 |
| Ornithine carbamoyltransferase (EC 2.1.3.3) | 3.64 | 2.41E−106 | 26,481 | 0.83 | 4.99E−08 | 9679 |
| Arginine/ornithine antiporter ArcD | 3.64 | 2.05E−113 | 5413 | 0.77 | 3.52E−10 | 1437 |
| Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24) | 3.59 | 3.90E−152 | 39,673 | 0.04 | 8.67E−01 | 4886 |
| Phage lysin, glycosyl hydrolase, family 25 | 3.57 | 2.01E−17 | 1011 | 5.13 | 2.13E−18 | 1903 |
| hypothetical protein | 3.49 | 1.36E−161 | 1036 | – | – | – |
| Purine nucleoside phosphorylase (EC 2.4.2.1) | 3.48 | 9.00E−58 | 3140 | − 0.43 | 5.93E−02 | 2133 |
| Aggregation promoting factor | 2.80 | 7.03E−15 | 37,746 | 5.84 | 1.04E−25 | 22,462 |
| Phage lysin, glycosyl hydrolase, family 25 | 3.57 | 2.01E−17 | 1011 | 5.13 | 2.13E−18 | 1903 |
| lipoprotein precursor (putative) | 3.16 | 7.83E−54 | 990 | 4.08 | 3.75E−79 | 1987 |
| NLP/P60 family protein | 1.40 | 7.51E−14 | 1243 | 3.85 | 4.25E−45 | 5309 |
| NrdR-regulated deoxyribonucleotide transporter, PnuC-like | 4.67 | 5.67E−148 | 2208 | 3.22 | 6.92E−51 | 1278 |
| Phage lysin, glycosyl hydrolase, family 25 | 2.90 | 3.22E−122 | 22,673 | 2.88 | 3.06E−47 | 12,481 |
| Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily | 2.20 | 2.22E−44 | 7114 | 2.87 | 2.95E−47 | 8280 |
| Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1) | 4.41 | 1.19E−258 | 79,074 | 2.86 | 1.08E−108 | 52,006 |
| peptidoglycan lytic protein P45 | 2.32 | 5.18E−79 | 4020 | 2.85 | 2.14E−86 | 3066 |
| Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1) | 3.10 | 1.75E−67 | 57,256 | 2.80 | 2.80E−138 | 50,915 |
| Streptococcal hemagglutinin protein | 2.60 | 8.15E−24 | 2848 | 2.72 | 5.81E−22 | 3186 |
| Bifunctional autolysin Atl/N-acetylmuramoyl-l-alanine amidase (EC 3.5.1.28)/ endo-beta-N-acetylglucosaminidase (EC 3.2.1.96) | 1.64 | 1.61E−22 | 11,980 | 2.69 | 1.48E−38 | 10,461 |
| Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1) | 2.52 | 2.47E−108 | 55,842 | 2.64 | 5.47E−118 | 46,441 |
| Aggregation promoting factor | 0.50 | 5.01E−03 | 684 | 2.60 | 4.12E−20 | 698 |
| Aggregation promoting factor | 0.72 | 3.32E−03 | 1607 | 2.59 | 2.95E−24 | 890 |
| tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmE | 2.45 | 5.57E−70 | 3144 | 2.56 | 8.51E−48 | 3573 |
| DNA topoisomerase IV subunit A (EC 5.99.1.3) | 1.39 | 8.11E−56 | 5955 | 2.49 | 6.22E−90 | 13,461 |
| Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NADP +] (EC 1.2.1.79) | 2.73 | 5.84E−131 | 4798 | 2.37 | 2.51E−66 | 4039 |
| tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmG | 2.63 | 1.75E−52 | 4279 | 2.36 | 3.10E−31 | 5697 |
| Uronate isomerase (EC 5.3.1.12) | 4.90 | 4.71E−139 | 10,468 | 2.29 | 4.39E−29 | 1311 |