Figure 2. Machine learning‐based functional screening identified key kinase drivers of the NTD‐stimulated cytokine release in PBMCs.

• A
  A schematic showing the process of the machine learning‐based functional screening.
• B
  A heatmap showing changes in the indicated cytokines in response to treatment of PBMCs with 35 "tool" kinase inhibitors at 500 nM as the training set. Cytokines released in the media were measured by Luminex and normalized to DMSO control.
• C
  Validation of the predicted kinases as drivers of cytokine release by siRNAs in THP1 cells. Cytokine release was measured by Luminex and normalized to scrambled siRNA.