PBMCs and cell lines
|
THP1 |
ATCC |
TIB‐202 |
Raw264.7 |
ATCC |
TIB‐71 |
PBMCs from healthy donors |
Bloodworks NW, Seattle, WA |
N/A |
PBMCs from COVID patients |
The Seattle Vaccine Trials Unit |
N/A |
Recombinant purified proteins
|
Full‐length S1 protein SARS‐CoV‐2 Wuhan |
Ray Biotech Life |
230‐30161 |
RBD SARS‐CoV‐2 Wuhan |
Ray Biotech Life |
230‐30162 |
NTD SARS‐CoV‐2 Wuhan |
Leinco Technologies Inc |
S853 |
NTD SARS‐CoV‐2 Wuhan |
Acro Biosystems |
S1D‐C52H6 |
NTD SARS‐CoV‐2 U.K. (B.1.1.7) |
Acro Biosystems |
S1D‐C52Hd |
NTD SARS‐CoV‐2 South Africa (B.1.351) |
Acro Biosystems |
S1D‐C52Hc |
NTD SARS‐CoV‐2 Delta (B.1.651.2) |
Acro Biosystems |
S1D‐C52Hf |
S1 protein HCoV‐229E |
Sino Biological Inc. |
40605‐V08B |
S1 protein HCoV‐OC43 |
Sino Biological Inc. |
40607‐V08H1 |
Chemicals and biologics
|
Ponatinib |
Selleck Chemicals |
S1490 |
Dexamethasone |
Selleck Chemicals |
S1322 |
Baricitinib |
Selleck Chemicals |
S2851 |
SB218078 |
Tocris Bioscience |
2560 |
Cobimetinib |
Cayman Chemicals |
19563 |
AT9283 |
Selleck Chemicals |
S1134 |
Sunitinib |
Cayman Chemicals |
13159 |
Bosutinib |
Tocris Bioscience |
4361 |
TAE‐684 |
Cayman Chemicals |
17670 |
GSK‐650394 |
NCGC00250410 |
NCATS (NCGC) |
Axitinib |
Selleck Chemicals |
S1005 |
Phorbol 12‐myristate 13‐acetate |
LC Laboratories |
P‐1680 |
Lipopolysaccharides |
Sigma Aldrich |
L2630 |
Cell culture reagents
|
RPMI1640 |
Fisher Scientific |
11875135 |
DMEM |
Fisher Scientific |
MT10017CM |
FBS |
Gibco |
26140‐079 |
Penn Strep. |
Fisher Scientific |
15140163 |
Sodium pyruvate |
Fisher Scientific |
BW13115E |
Luminex capture antibodies
|
Mouse GMCSF |
BioLegend |
505408 |
Mouse IL‐6 |
BD Biosciences |
554398 |
Mouse TNFα |
BioLegend |
510804 |
Human IL1 β |
R&D Systems |
MAB601 |
Human IL‐6 |
R&D Systems |
MAB206 |
Human IL‐8 |
Fisher Scientific |
M801 |
Human IL‐10 |
Fisher Scientific |
M010 |
Human MCP1 |
BD Biosciences |
555055 |
Human GMCSF |
BD Biosciences |
554502 |
Human TNFα |
BD Biosciences |
551220 |
Luminex detection antibodies
|
Mouse GMCSF |
BioLegend |
505502 |
Mouse IL‐6 |
BioLegend |
504602 |
Mouse TNFα |
BioLegend |
506312 |
Human IL1 β |
R&D Systems |
BAF201 |
Human IL‐6 |
R&D Systems |
BAF206 |
Human IL‐8 |
Fisher Scientific |
M802B |
Human IL‐10 |
BioLegend |
501502 |
Human MCP1 |
BioLegend |
502609 |
Human GMCSF |
BD Biosciences |
554505 |
Human TNFα |
BioLegend |
502904 |
AIM assay reagents
|
Human Serum AB |
Gemini Bio |
100‐512 |
Cytomegalovirus control peptide pool |
AnaSpec |
AS‐62339 |
Staphylococcal enterotoxin B |
Calbiochem |
11100‐45‐1 |
Live/Dead Aqua |
Invitrogen |
L34957 |
Cell Staining Buffer |
BioLegend |
420201 |
Human TruStain FcX |
BioLegend |
422302 |
Brilliant Stain Buffer |
Fisher Scientific |
BDB563794 |
Paraformaldehyde |
Fisher Scientific |
50‐980‐487 |
AIM assay antibodies
|
CD40 |
Miltenyi |
130‐094‐133 |
CD3 ECD |
Beckman Coulter |
IM270SU |
CD4 Alexa Fluor 488 |
BD Biosciences |
557695 |
CD8 PerCP eFluor 710 |
eBioscience |
46‐0087‐42 |
CD69 BV421 |
BD Biosciences |
562884 |
CD137 APC |
BD Biosciences |
550890 |
CD154 PE |
BD Biosciences |
555700 |
Small interfering RNA
|
si‐MAPK12 |
Dharmacon |
L‐003590‐00 |
si‐EPHA7 |
Dharmacon |
L‐003119‐00 |
si‐MAP3K8 |
Dharmacon |
L‐003511‐00 |
si‐PRKACG |
Dharmacon |
L‐004651‐00 |
si‐IRAK1 |
Dharmacon |
L‐004760‐00 |
si‐MAP3K3 |
Dharmacon |
L‐003301‐00 |
si‐JAK1 |
Dharmacon |
L‐003145‐00 |
si‐PTK2B |
Dharmacon |
L‐003165‐00 |
si‐MAPK14 |
Dharmacon |
L‐003512‐00 |
si‐PTK5 |
Dharmacon |
L‐003139‐00 |
si‐MAP4K2 |
Dharmacon |
L‐003587‐00 |
si‐STK17A |
Dharmacon |
L‐005377‐00 |
si‐CAMKK2 |
Dharmacon |
L‐004842‐00 |
si‐Scrambled |
Dharmacon |
D‐001810‐10 |
Oligonucleotides
|
qPCR primers |
Bio‐Rad |
|
Real time primers |
See Dataset EV2
|
|
Animals
|
|
|
C57BL/6J mice, male |
The Jackson Laboratory |
000664 |
Other reagents
|
CellTiter‐Glo 2.0 Cell Viability Assay |
Promega |
G9241 |
Lipofectamine RNAiMax |
Thermo Scientific |
13778150 |
RNeasy Mini Kit |
QIAGEN |
74104 |
RT2 First‐Strand Kit |
QIAGEN |
330411 |
SYBR Green Supermix |
Bio‐Rad |
1725274 |
Instrument
|
Luminex 200 instrument |
Luminex |
N/A |
Plate reader |
N/A |
N/A |
Bio‐Rad CFX384 thermocycler |
Bio‐Rad |
1855484 |
Northern Lights spectral flow cytometer |
Cytek |
N/A |
Software
|
GraphPad Prism 8.0 |
https://www.graphpad.com/
|
N/A |
SpectroFlo |
Cytek |
N/A |