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. 2021 Sep 6;17(9):e10426. doi: 10.15252/msb.202110426
Reagent/resource Reference or source Identifier or catalog number
PBMCs and cell lines
THP1 ATCC TIB‐202
Raw264.7 ATCC TIB‐71
PBMCs from healthy donors Bloodworks NW, Seattle, WA N/A
PBMCs from COVID patients The Seattle Vaccine Trials Unit N/A
Recombinant purified proteins
Full‐length S1 protein SARS‐CoV‐2 Wuhan Ray Biotech Life 230‐30161
RBD SARS‐CoV‐2 Wuhan Ray Biotech Life 230‐30162
NTD SARS‐CoV‐2 Wuhan Leinco Technologies Inc S853
NTD SARS‐CoV‐2 Wuhan Acro Biosystems S1D‐C52H6
NTD SARS‐CoV‐2 U.K. (B.1.1.7) Acro Biosystems S1D‐C52Hd
NTD SARS‐CoV‐2 South Africa (B.1.351) Acro Biosystems S1D‐C52Hc
NTD SARS‐CoV‐2 Delta (B.1.651.2) Acro Biosystems S1D‐C52Hf
S1 protein HCoV‐229E Sino Biological Inc. 40605‐V08B
S1 protein HCoV‐OC43 Sino Biological Inc. 40607‐V08H1
Chemicals and biologics
Ponatinib Selleck Chemicals S1490
Dexamethasone Selleck Chemicals S1322
Baricitinib Selleck Chemicals S2851
SB218078 Tocris Bioscience 2560
Cobimetinib Cayman Chemicals 19563
AT9283 Selleck Chemicals S1134
Sunitinib Cayman Chemicals 13159
Bosutinib Tocris Bioscience 4361
TAE‐684 Cayman Chemicals 17670
GSK‐650394 NCGC00250410 NCATS (NCGC)
Axitinib Selleck Chemicals S1005
Phorbol 12‐myristate 13‐acetate LC Laboratories P‐1680
Lipopolysaccharides Sigma Aldrich L2630
Cell culture reagents
RPMI1640 Fisher Scientific 11875135
DMEM Fisher Scientific MT10017CM
FBS Gibco 26140‐079
Penn Strep. Fisher Scientific 15140163
Sodium pyruvate Fisher Scientific BW13115E
Luminex capture antibodies
Mouse GMCSF BioLegend 505408
Mouse IL‐6 BD Biosciences 554398
Mouse TNFα BioLegend 510804
Human IL1 β R&D Systems MAB601
Human IL‐6 R&D Systems MAB206
Human IL‐8 Fisher Scientific M801
Human IL‐10 Fisher Scientific M010
Human MCP1 BD Biosciences 555055
Human GMCSF BD Biosciences 554502
Human TNFα BD Biosciences 551220
Luminex detection antibodies
Mouse GMCSF BioLegend 505502
Mouse IL‐6 BioLegend 504602
Mouse TNFα BioLegend 506312
Human IL1 β R&D Systems BAF201
Human IL‐6 R&D Systems BAF206
Human IL‐8 Fisher Scientific M802B
Human IL‐10 BioLegend 501502
Human MCP1 BioLegend 502609
Human GMCSF BD Biosciences 554505
Human TNFα BioLegend 502904
AIM assay reagents
Human Serum AB Gemini Bio 100‐512
Cytomegalovirus control peptide pool AnaSpec AS‐62339
Staphylococcal enterotoxin B Calbiochem 11100‐45‐1
Live/Dead Aqua Invitrogen L34957
Cell Staining Buffer BioLegend 420201
Human TruStain FcX BioLegend 422302
Brilliant Stain Buffer Fisher Scientific BDB563794
Paraformaldehyde Fisher Scientific 50‐980‐487
AIM assay antibodies
CD40 Miltenyi 130‐094‐133
CD3 ECD Beckman Coulter IM270SU
CD4 Alexa Fluor 488 BD Biosciences 557695
CD8 PerCP eFluor 710 eBioscience 46‐0087‐42
CD69 BV421 BD Biosciences 562884
CD137 APC BD Biosciences 550890
CD154 PE BD Biosciences 555700
Small interfering RNA
si‐MAPK12 Dharmacon L‐003590‐00
si‐EPHA7 Dharmacon L‐003119‐00
si‐MAP3K8 Dharmacon L‐003511‐00
si‐PRKACG Dharmacon L‐004651‐00
si‐IRAK1 Dharmacon L‐004760‐00
si‐MAP3K3 Dharmacon L‐003301‐00
si‐JAK1 Dharmacon L‐003145‐00
si‐PTK2B Dharmacon L‐003165‐00
si‐MAPK14 Dharmacon L‐003512‐00
si‐PTK5 Dharmacon L‐003139‐00
si‐MAP4K2 Dharmacon L‐003587‐00
si‐STK17A Dharmacon L‐005377‐00
si‐CAMKK2 Dharmacon L‐004842‐00
si‐Scrambled Dharmacon D‐001810‐10
Oligonucleotides
qPCR primers Bio‐Rad
Real time primers See Dataset EV2
Animals
C57BL/6J mice, male The Jackson Laboratory 000664
Other reagents
CellTiter‐Glo 2.0 Cell Viability Assay Promega G9241
Lipofectamine RNAiMax Thermo Scientific 13778150
RNeasy Mini Kit QIAGEN 74104
RT2 First‐Strand Kit QIAGEN 330411
SYBR Green Supermix Bio‐Rad 1725274
Instrument
Luminex 200 instrument Luminex N/A
Plate reader N/A N/A
Bio‐Rad CFX384 thermocycler Bio‐Rad 1855484
Northern Lights spectral flow cytometer Cytek N/A
Software
GraphPad Prism 8.0 https://www.graphpad.com/ N/A
SpectroFlo Cytek N/A