Reagent/resource	Reference or source	Identifier or catalog number
PBMCs and cell lines
THP1	ATCC	TIB‐202
Raw264.7	ATCC	TIB‐71
PBMCs from healthy donors	Bloodworks NW, Seattle, WA	N/A
PBMCs from COVID patients	The Seattle Vaccine Trials Unit	N/A
Recombinant purified proteins
Full‐length S1 protein SARS‐CoV‐2 Wuhan	Ray Biotech Life	230‐30161
RBD SARS‐CoV‐2 Wuhan	Ray Biotech Life	230‐30162
NTD SARS‐CoV‐2 Wuhan	Leinco Technologies Inc	S853
NTD SARS‐CoV‐2 Wuhan	Acro Biosystems	S1D‐C52H6
NTD SARS‐CoV‐2 U.K. (B.1.1.7)	Acro Biosystems	S1D‐C52Hd
NTD SARS‐CoV‐2 South Africa (B.1.351)	Acro Biosystems	S1D‐C52Hc
NTD SARS‐CoV‐2 Delta (B.1.651.2)	Acro Biosystems	S1D‐C52Hf
S1 protein HCoV‐229E	Sino Biological Inc.	40605‐V08B
S1 protein HCoV‐OC43	Sino Biological Inc.	40607‐V08H1
Chemicals and biologics
Ponatinib	Selleck Chemicals	S1490
Dexamethasone	Selleck Chemicals	S1322
Baricitinib	Selleck Chemicals	S2851
SB218078	Tocris Bioscience	2560
Cobimetinib	Cayman Chemicals	19563
AT9283	Selleck Chemicals	S1134
Sunitinib	Cayman Chemicals	13159
Bosutinib	Tocris Bioscience	4361
TAE‐684	Cayman Chemicals	17670
GSK‐650394	NCGC00250410	NCATS (NCGC)
Axitinib	Selleck Chemicals	S1005
Phorbol 12‐myristate 13‐acetate	LC Laboratories	P‐1680
Lipopolysaccharides	Sigma Aldrich	L2630
Cell culture reagents
RPMI1640	Fisher Scientific	11875135
DMEM	Fisher Scientific	MT10017CM
FBS	Gibco	26140‐079
Penn Strep.	Fisher Scientific	15140163
Sodium pyruvate	Fisher Scientific	BW13115E
Luminex capture antibodies
Mouse GMCSF	BioLegend	505408
Mouse IL‐6	BD Biosciences	554398
Mouse TNFα	BioLegend	510804
Human IL1 β	R&D Systems	MAB601
Human IL‐6	R&D Systems	MAB206
Human IL‐8	Fisher Scientific	M801
Human IL‐10	Fisher Scientific	M010
Human MCP1	BD Biosciences	555055
Human GMCSF	BD Biosciences	554502
Human TNFα	BD Biosciences	551220
Luminex detection antibodies
Mouse GMCSF	BioLegend	505502
Mouse IL‐6	BioLegend	504602
Mouse TNFα	BioLegend	506312
Human IL1 β	R&D Systems	BAF201
Human IL‐6	R&D Systems	BAF206
Human IL‐8	Fisher Scientific	M802B
Human IL‐10	BioLegend	501502
Human MCP1	BioLegend	502609
Human GMCSF	BD Biosciences	554505
Human TNFα	BioLegend	502904
AIM assay reagents
Human Serum AB	Gemini Bio	100‐512
Cytomegalovirus control peptide pool	AnaSpec	AS‐62339
Staphylococcal enterotoxin B	Calbiochem	11100‐45‐1
Live/Dead Aqua	Invitrogen	L34957
Cell Staining Buffer	BioLegend	420201
Human TruStain FcX	BioLegend	422302
Brilliant Stain Buffer	Fisher Scientific	BDB563794
Paraformaldehyde	Fisher Scientific	50‐980‐487
AIM assay antibodies
CD40	Miltenyi	130‐094‐133
CD3 ECD	Beckman Coulter	IM270SU
CD4 Alexa Fluor 488	BD Biosciences	557695
CD8 PerCP eFluor 710	eBioscience	46‐0087‐42
CD69 BV421	BD Biosciences	562884
CD137 APC	BD Biosciences	550890
CD154 PE	BD Biosciences	555700
Small interfering RNA
si‐MAPK12	Dharmacon	L‐003590‐00
si‐EPHA7	Dharmacon	L‐003119‐00
si‐MAP3K8	Dharmacon	L‐003511‐00
si‐PRKACG	Dharmacon	L‐004651‐00
si‐IRAK1	Dharmacon	L‐004760‐00
si‐MAP3K3	Dharmacon	L‐003301‐00
si‐JAK1	Dharmacon	L‐003145‐00
si‐PTK2B	Dharmacon	L‐003165‐00
si‐MAPK14	Dharmacon	L‐003512‐00
si‐PTK5	Dharmacon	L‐003139‐00
si‐MAP4K2	Dharmacon	L‐003587‐00
si‐STK17A	Dharmacon	L‐005377‐00
si‐CAMKK2	Dharmacon	L‐004842‐00
si‐Scrambled	Dharmacon	D‐001810‐10
Oligonucleotides
qPCR primers	Bio‐Rad
Real time primers	See Dataset EV2
Animals
C57BL/6J mice, male	The Jackson Laboratory	000664
Other reagents
CellTiter‐Glo 2.0 Cell Viability Assay	Promega	G9241
Lipofectamine RNAiMax	Thermo Scientific	13778150
RNeasy Mini Kit	QIAGEN	74104
RT2 First‐Strand Kit	QIAGEN	330411
SYBR Green Supermix	Bio‐Rad	1725274
Instrument
Luminex 200 instrument	Luminex	N/A
Plate reader	N/A	N/A
Bio‐Rad CFX384 thermocycler	Bio‐Rad	1855484
Northern Lights spectral flow cytometer	Cytek	N/A
Software
GraphPad Prism 8.0	https://www.graphpad.com/	N/A
SpectroFlo	Cytek	N/A